A simple and fast method was used to determine 1,1'- (2,2,2-trichloroethylene) - bis (4-chlorobenzene) (DDT) and 1,1'- (2,2-dichloroethylidene) - bis (4-chlorobenzene) (DDE) in blood serum.
Serum samples pre-treated with formic acid were extracted with n-hexane and determined by gas chromatography (GC-ECD), using PTE-5 capillary column.
Detection limits for DDT and DDE were 0.5 ppb.
Recovery with a fortified pooled sample at 1 ppb level was 107.0% for DDT and 106.0% for DDE.
At 10 ppb level, the recoveries for DDT and DDE were 96.1% and 92.7%, respectively.
Eighty-nine random blood samples collected from volunteers were analyzed.
The geometric mean (GM) serum level of DDT was 1.9 ppb (0.2-8.9 ppb) and that of DDE was 10.8 ppb (1.5-88.1 ppb).
There was a positive correlation between DDE and DDT level (r=0.33, P<0.01).
The serum DDE level was positively correlated with age (r=0.49, P<0.01) and DDT x Age (r=0.62, P<0.01).
No correlation was observed between DDT and age.
These results suggest that DDE, a metabolite of DDT, is cumulative in the body.
Therefore blood DDE could be used as a cumulative exposure marker for DDT, whereas blood DDT may be used to reflect its recent exposure.
Mots-clés Pascal : Pesticide, Produit dégradation, Polluant, Environnement, Taux, Sérum, Liquide biologique, Sang, Analyse quantitative, Epidémiologie, Singapour, Asie, Marqueur biologique, Temps exposition, Homme, Chlore Composé organique, Organochloré, DDE, DDT
Mots-clés Pascal anglais : Pesticides, Degradation product, Pollutant, Environment, Rate, Serum, Biological fluid, Blood, Quantitative analysis, Epidemiology, Singapore, Asia, Biological marker, Exposure time, Human, Chlorine Organic compounds, Organochlorine compounds, DDE, DDT
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0131808
Code Inist : 002B03M01. Création : 22/06/1998.