Albumin and hemoglobin adducts as biomarkers of exposure to styrene in fiberglass-reinforced-plastics workers.
The purpose of this work was to compare levels of styrene-7,8-oxide (SO) adducts of albumin (Alb) and hemoglobin (Hb) with those of two urinary metabolites of styrene, mandelic acid (MA) and phenylglyoxylic acid (PGA), among workers exposed to styrene in the reinforced-plastics industry and in unexposed subjects.
We also wished to determine whether cigarette smoking influenced adduct levels among these subjects.
A group of 22 male workers was selected on basis of an expectedly high level of exposure to styrene, and a group of 15 controls was selected from hospital blood donors and hospital staff.
In the exposed group, MA and PGA were quantified by high-performance liquid chromatography (HPLC) analysis of urine samples collected prior to the work shift.
The SO adducts were cleaved from cysteine residues by reaction with Raney nickel to give 1-phenylethanol (I-PE) and 2-phenylethanol (2-PE), which, after derivatization, were measured using gas chromatography-mass spectrometry (GC-MS) in the negative-chemical-ionization (NCI) mode.
The estimated mean levels ofMA and MA+PGA were 74 and 159 mg/g creatinine, respectively.
Using the levels of urinary metabolites, an average styrene concentration of about 100 mg/m3 in the workplace air was estimated.
The mean levels of 2-PE and 1-PE adducts in exposed workers were 2.84 and 0.60 nmol/g Alb and 5.44 and 0.43 nmol/g Hb, respectively. (...)
Mots-clés Pascal : Styrène, Exposition professionnelle, Industrie transformation plastique, Plastique renforcé fibre, Fibre verre, Surveillance biologique, Adduit moléculaire, Albumine, Hémoglobine, Métabolite, Urine, Homme, Marqueur biologique, Médecine travail
Mots-clés Pascal anglais : Styrene, Occupational exposure, Plastics processing industry, Fibre reinforced plastics, Glass fiber, Biological monitoring, Molecular adduct, Albumin, Hemoglobin, Metabolite, Urine, Human, Biological marker, Occupational medicine
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0121753
Code Inist : 002B03L06. Création : 22/06/1998.