Assessment of potential damage to DNA in urine of coke oven workers : an assay of unscheduled DNA synthesis.
Objectives-A study was conducted in coke oven workers to evaluate the biological consequences of the exposure of these workers, particularly production of potential genotoxic factors.
Methods-60 coke oven workers and 40 controls were recruited in the same iron and steel works.
Exposure to polycyclic aromatic hydrocarbons (PAHs) was assessed by job and measurement of 1-hydroxypyrene (1OHP) in urine samples.
An unscheduled DNA synthesis assay was performed on rat pleural mesothelial cells used as a test system to evaluate the effect of the workers'filtered urine on the DNA repair capacity of rat cells to determine whether DNA damaging agents are present in the urine of these workers.
Urinary concentrations of 1OHP ranged from 0.06 to 24.2 (mean (SD) 2.1 (3.6)) mumol/mol creatinine in exposed coke oven workers, and from 0.01 to 0.9 in controls (0.12 (0.15)). These high concentrations in coke oven workers reflected recent exposure to PAHs and were in agreement with the assessment of exposure by job.
No significant difference was found between coke oven workers and controls in the DNA repair level of rat cells treated with urine samples.
However, the rat cell repair capacity decreased with increasing 1OHP concentrations in the exposed population (r=-0.28, P<0.05).
Conclusions-As high concentrations of 1OHP were found in the urine of some workers, a more stringent control of exposures to PAHs in the workplace is required. (...)
Mots-clés Pascal : Four coke, Exposition professionnelle, Médecine travail, Industrie métallurgique, Toxicité, Homme, Composé aromatique polycyclique, Surveillance biologique, Urine, Liquide biologique, DNA, Synthèse DNA non programmée, Carcinogène
Mots-clés Pascal anglais : Coke oven, Occupational exposure, Occupational medicine, Metallurgical industry, Toxicity, Human, Polycyclic aromatic compound, Biological monitoring, Urine, Biological fluid, DNA, Unscheduled DNA synthesis, Carcinogen
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0073699
Code Inist : 002B03L06. Création : 14/05/1998.