The study was carried out to determine the incidence of Salmonella spp. in sewage and semi-urban waste water treated by the pond oxidation system, using a Salmonella-specific DNA probe.
The Salmonella-specific DNA probe was labelled following the digoxigenin (DIG) standard random primed DNA labelling technique.
For the detection system, both the DIG calorimetric and chemiluminescent detection systems were used.
When a total of 803 samples, collected from the four oxidation ponds A, B, C, and D, the irrigation water, overflow polluted water, the stream and the borehole, were screened, using a Salmonella DNA probe, 96,4% of the samples from oxidation Pond A, 51% from Pond B, 25.0% from Pond C, and 71.0% from Pond D, were Salmonella positive.
The results also showed that 44.1% of the water samples from irrigation water, 69.9% of overflow polluted water samples and 83% of stream water samples collected from pools were positive for Salmonella presence.
An overall positivity rate of 36% on all the samples tested, was observed.
Significantly, no Salmonella positive samples were observed when water samples were collected from the one borehole which serviced the villagers living around the oxidation ponds.
It may be concluded from these results that the treatment of sewage and semi-urban waste water, using the oxidation pond methods at the University of the North, did not efficiently remove pathogenic bacteria such as Salmonella from treated effluent. (...)
Mots-clés Pascal : Epuration eau usée, Station épuration, Milieu urbain, Oxydation, Effluent, Contrôle microbiologique, Salmonella, Enterobacteriaceae, Bactérie, Contrôle qualité, Détection, Contamination biologique, Méthode étude, Hybridation moléculaire, DNA, République Sud Africaine, Afrique
Mots-clés Pascal anglais : Waste water purification, Sewage treatment plant, Urban environment, Oxidation, Effluent, Microbiological testing, Salmonella, Enterobacteriaceae, Bacteria, Quality control, Detection, Biological contamination, Investigation method, Molecular hybridization, DNA, South Africa, Africa
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0071837
Code Inist : 002B30A02B. Création : 14/05/1998.