IAMFES Annual Meeting. Seattle, WA, USA, 1996/06/30.
A prospective study was carried out in collaboration with two children's hospitals in Würzburg, Germany to assess the incidence and clinical manifestations of infections due to Shiga toxin-producing Escherichia coli (STEC) in children.
Between 1991 and 1995, stool samples from 2788 children with enteritis were investigated for the occurrence of STEC.
STEC cultures from stools were screened using PCR with primers complementary to Shiga toxin 1 (Stx 1) and Shiga toxin 2 (Stx2) genes.
PCR-positive samples were further subjected to colony blot hybridization and probe positive colonies were serotyped and analyzed for the presence of virulence genes.
There was an increase in the incidence of STEC infections from 0.4% in 1991 to 2.8% in 1994.
In 1995 the number of infections remained nearly unchanged (2.5%). Infection with STEC was associated with painful nonbloody diarrhea in most patients.
Among the 35 patients in this study with stools containing STEC, only 9 (25.7%) had 0157 colonies of which 3 (8.6%) were O157 : H7 and 6 (17.1%) were sorbitol-fermenting O157 : H-In an additional study in 1994/1995, STEC etiology in 88 patients with HUS from Germany was confirmed in our laboratories by culture of STEC from stools, and in 20 additional HUS cases by serological analysis.
Of the strains from stools of HUS patients, 78% belonged to serogroup 0157.
The most frequently isolated non-O157 serogroups were 026 and 0111. (...)
Mots-clés Pascal : Escherichia coli, Enterobacteriaceae, Bactérie, Bactériose, Infection, Hémolyse urémie, Epidémiologie, Allemagne, Europe, Diarrhée, Enfant, Homme, Sérotype, Insuffisance rénale, Appareil urinaire pathologie, Hémopathie, Anémie hémolytique, Rein pathologie, Appareil digestif pathologie, Intestin pathologie, Toxine type Shiga
Mots-clés Pascal anglais : Escherichia coli, Enterobacteriaceae, Bacteria, Bacteriosis, Infection, Hemolytic uremic syndrome, Epidemiology, Germany, Europe, Diarrhea, Child, Human, Serotype, Renal failure, Urinary system disease, Hemopathy, Hemolytic anemia, Kidney disease, Digestive diseases, Intestinal disease, Shiga-like toxin
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Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0069767
Code Inist : 002B05B02F. Création : 14/05/1998.