Analysis of epidemic Pseudomonas aeruginosa isolates by isoelectric focusing of pyoverdine and RAPD-PCR : modern tools for an integrated anti-nosocomial infection strategy in burn wound centres.
The Gram-negative bacterium Pseudomonas aeruginosa is an important life-threatening nosocomial pathogen in burn units.
In this study we analysed epidemic P. aeruginosa isolates from patients and their hospital environment using two new molecular techniques in order to establish strain relatedness for epidemiological purposes.
One technique was pyoverdine typing by isoelectric focusing (PVLJ-IEF) and the other was a genomic PCR-based fingerprinting technique called random amplification of polymorphic DNA actually referred to as RAPD-PCR.
The described short epidemic (6 weeks) included 37 consecutive isolates from 9 different patients as well as two environmental isolates recovered, at the same time, from one of the hydrotherapy facilities.
Only two of the three known pyoverdine types of P. aeruginosa could be found.
Type I was absent while type II represented 49 per cent and type III, 51 per cent of the isolates.
The two consecutive isolates from the environment were both of type III.
The RAPD-PCR fingerprinting discriminated four patterns.
Profile 1 represented 60 per cent ; profile 2,34 per cent ; and profiles 3 and 4 only 3 per cent of the isolates respectively.
The environmental isolates also had a RAPD-PCR 1 profile, arguing for the hydrotherapy facility as a possible contamination source.
Prompt measures could prevent an outbreak.
Mots-clés Pascal : Brûlure, Infection nosocomiale, Pseudomonas aeruginosa, Pseudomonadaceae, Pseudomonadales, Bactérie, Antibiotique, Résistance traitement, Typologie, Réaction chaîne polymérase, Microbiologie, Epidémiologie, Homme, Prévention, Traumatisme, Soin intensif
Mots-clés Pascal anglais : Burn, Nosocomial infection, Pseudomonas aeruginosa, Pseudomonadaceae, Pseudomonadales, Bacteria, Antibiotic, Negative therapeutic reaction, Typology, Polymerase chain reaction, Microbiology, Epidemiology, Human, Prevention, Trauma, Intensive care
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0053086
Code Inist : 002B27B09. Création : 14/05/1998.