Background TDI-induced asthma exhibits clinical, functional and morphological similarities with allergen-induced asthma, suggesting that an immunological mechanism is involved in the sensitization to TDI.
In vitro studies using the technique of cloning lymphocytes demonstrated that a great proportion of T-cell clones derived from bronchial mucosa of subjects with TDI-induced asthma produced IL-5 and interferon-gamma, but not IL-4, upon in vitro stimulation.
Objectives To investigate in vivo the role of IL-4 and IL-5 on the inflammatory response of the bronchial mucosa to TDI in sensitized subjects, we performed a quantitative analysis of bronchial biopsies.
Methods We obtained bronchial biopsies from six subjects with TDI asthma 48 h after an asthmatic reaction induced by TDI challenge (challenged group), in six subjects with TDI asthma 1-4 weeks after the last exposure to TDI (chronic group), and in six non-asthmatic controls.
The number of eosinophils, mast cells, T-lymphocytes, and IL-4 and IL-5 protein positive cells was determined by immunohistochemistry in the area 100 mum beneath the epithelial basement membrane.
Results The characteristic increase of submucosal eosinophils, but not of mast cells and T-lymphocytes, was observed in the subjects with TDI-induced asthma when compared with controls.
No differences were detected between the two groups of asthmatics. (...)
Mots-clés Pascal : Asthme, Interleukine 4, Interleukine 5, Toluène dérivé, Diisocyanate organique, Inflammation, Bronche, Exploration immunologique, Médecine travail, Exposition professionnelle, Homme, Appareil respiratoire pathologie, Bronchopneumopathie obstructive, Cytokine
Mots-clés Pascal anglais : Asthma, Interleukin 4, Interleukin 5, Toluene derivatives, Organic diisocyanate, Inflammation, Bronchus, Immunological investigation, Occupational medicine, Occupational exposure, Human, Respiratory disease, Obstructive pulmonary disease, Cytokine
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0043010
Code Inist : 002B03L04. Création : 17/04/1998.