Pathophysiology of premature skin aging induced by ultraviolet light.
Background Long-term exposure to ultraviolet irradiation from sunlight causes premature skin aging (photoaging), characterized in part by wrinkles, altered pigmentation, and loss of skin tone.
Photoaged skin displays prominent alterations in the collagenous extracellular matrix of connective tissue.
We investigated the role of matrix-degrading metalloproteinases, a family of proteolytic enzymes, as mediators of collagen damage in photoaging.
Methods We studied 59 whites (33 men and 26 women, ranging in age from 21 to 58 years) with light-to-moderate skin pigmentation, none of whom had current or prior skin disease.
Only some of the participants were included in each of the studies.
We irradiated their buttock skin with fluorescent ultraviolet lights under standard conditions and obtained skin samples from irradiated and nonirradiated areas by keratome or punch biopsy.
In some studies, tretinoin and its vehicle were applied to skin under occlusion 48 hours before ultraviolet irradiation.
The expression of matrix metalloproteinases was determined by in situ hybridization, immunohistology, and in situ zymography.
Irradiation-induced degradation of skin collagen was measured by radioimmunoassay of soluble cross-linked telopeptides.
The protein level of tissue inhibitor of matrix metalloproteinases type 1 was determined by Western blot analysis. (...)
Mots-clés Pascal : Irradiation UV, Exposition, Peau, Vieillissement accéléré, Métalloprotéine, Activité enzymatique, Santé et environnement, Physiopathologie, Homme
Mots-clés Pascal anglais : Ultraviolet irradiation, Exposure, Skin, Artificial ageing, Metalloproteins, Enzymatic activity, Health and environment, Pathophysiology, Human
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0042896
Code Inist : 002B30A02A. Création : 17/04/1998.