Mutations of the p53 gene are related to development of human cancers and their frequencies and spectra, the latter representing fingerprints left by carcinogens, provide information about the molecular epidemiology of the disease.
Prostate cancer is the most common neoplasm in American males and although its incidence is still relatively low in Japanese people, it has recently been increasing with the westernization of life style.
To assess the frequency and spectrum of p53 gene mutations in Japanese prostate cancers, we examined a series of 90 lesions using polymerase chain reaction (PCR) - single-strand conformation polymorphism (SSCP) analysis.
The patients'mean age was 69.3 years (range 57-87).
Of the total, six were well-34 moderately-and 50 poorly-differentiated adenocarcinomas, and the median Gleason score was 7.9. Eleven of the 90 cases (12%) had mutations in exons 2-11 of the p53 gene : none of the five clinical-stage A, one of 25 stage B (4%), three of 35 stage C (9%) and seven of 25 stage D (28%) cancers.
The correlation with an advanced stage was statistically significant.
One insertion and 10 base pair substitutions were encountered, comprising six transversions (55%) and four transitions (36%). Two of the latter involved methylated cytosine-guanine (CpG). (...)
Mots-clés Pascal : Homme, Japon, Asie, Amérique, Tumeur maligne, Prostate, Etude comparative, Mutation, Gène TP53, Prévalence, Epidémiologie moléculaire, Europe, Variation géographique, Mode de vie, Appareil génital mâle pathologie, Appareil urinaire pathologie, Prostate pathologie
Mots-clés Pascal anglais : Human, Japan, Asia, America, Malignant tumor, Prostate, Comparative study, Mutation, TP53 Gene, Prevalence, Molecular epidemiology, Europe, Geographical variation, Life habit, Male genital diseases, Urinary system disease, Prostate disease
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 98-0007656
Code Inist : 002B14D02. Création : 17/04/1998.