DNA restriction fragments length polymorphism of Mycobacterium tuberculosis isolates in Pisa, Italy.
A total of 60 Mycobacterium tuberculosis strains isolated in the area of Pisa, Italy, over a period from April 1993 to December 1995, were analyzed for the IS6110-based restriction fragments length polymorphism (RFLP).
Isolates were found to show a great heterogeneity and only few isolates shared identical DNA banding patterns.
In particular, 55 distinct IS6110 patterns were found (average number of isolates per pattern : 1.09) and only 9 strains (15%) occurred in 4 clusters of 2-3 identical clones.
Computer analysis of genetic similarities among the strains revealed a family of 17 isolates including the clustered clones implicated in recently acquired infections.
No correlation was found between the RFLP DNA patterns of the isolates and drug susceptibility.
Of the 5 isolates from immigrants only one showed abnormal DNA fingerprinting.
Our data indicate that the patterns of M. tuberculosis isolates in Pisa area are comparable to those of countries with low-prevalence TB and that a low level of TB transmission occurs in this area.
Mots-clés Pascal : Mycobacterium tuberculosis, Mycobacteriaceae, Mycobacteriales, Actinomycetes, Bactérie, Isolat clinique, Homme, Polymorphisme longueur fragment restriction, Sérotypie, Tuberculose, Mycobactériose, Bactériose, Infection, Epidémiologie moléculaire, Italie, Europe, Biologie moléculaire, Génétique
Mots-clés Pascal anglais : Mycobacterium tuberculosis, Mycobacteriaceae, Mycobacteriales, Actinomycetes, Bacteria, Clinical isolate, Human, Restriction fragment length polymorphism, Serotyping, Tuberculosis, Mycobacterial infection, Bacteriosis, Infection, Molecular epidemiology, Italy, Europe, Molecular biology, Genetics
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0548871
Code Inist : 002B05B02E. Création : 24/03/1998.