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  1. DNA restriction fragments length polymorphism of Mycobacterium tuberculosis isolates in Pisa, Italy.

    Article - En anglais

    A total of 60 Mycobacterium tuberculosis strains isolated in the area of Pisa, Italy, over a period from April 1993 to December 1995, were analyzed for the IS6110-based restriction fragments length polymorphism (RFLP).

    Isolates were found to show a great heterogeneity and only few isolates shared identical DNA banding patterns.

    In particular, 55 distinct IS6110 patterns were found (average number of isolates per pattern : 1.09) and only 9 strains (15%) occurred in 4 clusters of 2-3 identical clones.

    Computer analysis of genetic similarities among the strains revealed a family of 17 isolates including the clustered clones implicated in recently acquired infections.

    No correlation was found between the RFLP DNA patterns of the isolates and drug susceptibility.

    Of the 5 isolates from immigrants only one showed abnormal DNA fingerprinting.

    Our data indicate that the patterns of M. tuberculosis isolates in Pisa area are comparable to those of countries with low-prevalence TB and that a low level of TB transmission occurs in this area.

    Mots-clés Pascal : Mycobacterium tuberculosis, Mycobacteriaceae, Mycobacteriales, Actinomycetes, Bactérie, Isolat clinique, Homme, Polymorphisme longueur fragment restriction, Sérotypie, Tuberculose, Mycobactériose, Bactériose, Infection, Epidémiologie moléculaire, Italie, Europe, Biologie moléculaire, Génétique

    Mots-clés Pascal anglais : Mycobacterium tuberculosis, Mycobacteriaceae, Mycobacteriales, Actinomycetes, Bacteria, Clinical isolate, Human, Restriction fragment length polymorphism, Serotyping, Tuberculosis, Mycobacterial infection, Bacteriosis, Infection, Molecular epidemiology, Italy, Europe, Molecular biology, Genetics

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 97-0548871

    Code Inist : 002B05B02E. Création : 24/03/1998.