Metabolism of arsenic after acute occupational arsine intoxication.
Among the elements of toxicological relevance, inorganic arsenic (As) probably exhibits the most complex metabolism, and we deemed it interesting to identify and quantify the different As species excreted after an occupational acute intoxication with arsine.
For this purpose total As and five As species were determined using an hybrid analytical method coupling liquid chromatography with inductively coupled plasma mass spectrometry.
The highest urinary elimination of total As was observed in the first 5 d after admission.
The As species mostly excreted were monomethylarsonate (MMA), dimethylarsinate (DMA), As3+arsenobetaine (AsB), and to a lesser extent As5+The amount of AsB excreted in urine by the subject does not appear to be completely justified by AsB intake through food.
Arsenic is excreted mainly via the urine with a clearance of 7.8 ml/h/kg and follows a triphasic model with periods of 28 h, 59 h, and 9 d, respectively.
The evidence that DMA excretion culminates after a few days, when the excretion of the inorganic form is substantially reduced (while that of MMA is still elevated), seems to confirm the existence of two successive methylating enzyme activities.
Furthermore, the elimination rate of As from blood follows a three-phase model and the half-lives of different species vary from about 27 to 86 h with the following gradient As5+
Mots-clés Pascal : Toxicocinétique, Cinétique, Métabolisme, Elimination, Intoxication, Inhalation, Homme, Exposition professionnelle, Médecine travail, Arsenic Composé, Arsine, Urine, Liquide biologique, Sang, Spectrométrie ICP, Méthode couplée, Chromatographie HPLC
Mots-clés Pascal anglais : Toxicokinetics, Kinetics, Metabolism, Elimination, Poisoning, Inhalation, Human, Occupational exposure, Occupational medicine, Arsenic Compounds, Arsine, Urine, Biological fluid, Blood, Inductive coupling plasma spectrometry, Coupled method, HPLC chromatography
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0548832
Code Inist : 002B03L05. Création : 24/03/1998.