A guinea pig intratracheal test was developed to assess the respiratory allergenicity of enzymes used in the detergent industry.
Information gained from this test was used in a process for setting operational exposure guidelines to protect worker health.
Mixtures of enzyme proteins were given to guinea pigs once per week for 10 weeks to determine whether there were interactions among enzymes that affected the induction of antibody responses to each enzyme in the mixture.
Passive cutaneous anaphylaxis antibody titers against each enzyme were measured in sera.
Mixtures of two or three enzymes always consisted of a protease (Alcalase, Savinase ; Novo Industri A/S) with an alpha-amylase (Termamyl ; Novo Industri A/S), a lipase (Lipolase ; Novo Industri A/S), or both.
Control animals were exposed to single enzymes.
The antibody titers to Termamyl and Lipolase were significantly greater in animals dosed with the protease-containing mixtures as compared with control animals dosed with a single enzyme.
Antibody titers to the protease were unchanged in the presence of additional enzymes in the mixture.
Complete inactivation of protease activity abrogated the enhanced antibody response to Lipolase. (...)
Mots-clés Pascal : Allergie, Détergent, Peptidases, Hydrolases, Enzyme, Triacylglycerol lipase, Carboxylic ester hydrolases, Esterases, alpha-Amylase, O-Glycosidases, Glycosidases, Exposition professionnelle, Modèle animal, Cobaye, Rodentia, Mammalia, Vertebrata, Animal, Immunopathologie, Maladie professionnelle, Médecine travail
Mots-clés Pascal anglais : Allergy, Detergent, Peptidases, Hydrolases, Enzyme, Triacylglycerol lipase, Carboxylic ester hydrolases, Esterases, alpha-Amylase, O-Glycosidases, Glycosidases, Occupational exposure, Animal model, Guinea pig, Rodentia, Mammalia, Vertebrata, Animal, Immunopathology, Occupational disease, Occupational medicine
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0534541
Code Inist : 002B06B. Création : 13/02/1998.