Objectives-The interaction of benzo (a) pyrene with serum albumin was measured in an attempt to identify the actual exposure and to evaluate albumin adduct measurements as biomarkers for exposure monitoring.
Methods-Benzo (a) pyrene-diolepoxide (BPDE) - albumin adducts were measured by competitive enzyme linked immunosorbent assay (ELISA) in plasma of coke oven plant workers from three plants and from people living in a highly industrialised area of Silesia in Poland.
Due to the high air concentrations of polycyclic aromatic hydrocarbons (PAHs) in this area, a control group was selected from a rural non-industrialised area in Poland.
Breathing zone air measurements of PAHs were collected from some of the participants.
Coke oven plant workers and non-occupationally exposed people had similar concentrations of albumin adducts whereas the rural controls were significantly lower (2.74 fmol adducts/mug albumin (SEM 0.124)). The mean concentration of BPDE-albumin adduct in plasma of both the occupational and the environmental groups were significantly higher in the summer samples (4.34 fmol adducts/mug albumin (SEM 0.335) and 4.55 fmol adducts/mug albumin (SEM 0.296), respectively) than in the winter samples (3.06 fmol adducts/mug albumin (SEM 0.187) and 3.04 fmol adducts/mug albumin (SEM 0.184), respectively) even though the air measurements showed higher concentrations of PAHs in the winter. (...)
Mots-clés Pascal : Benzo[a]pyrène, Benzo[a]pyrène-7,8-diol(9,10-époxy-7,8,9,10-tétrahydro), Adduit moléculaire, Sérumalbumine, Composé aromatique polycyclique, Homme, Exposition professionnelle, Médecine travail, Pologne, Europe, Surveillance biologique, Marqueur biologique, Industrie, Plasma sanguin, Technique ELISA
Mots-clés Pascal anglais : Molecular adduct, Serum albumin, Polycyclic aromatic compound, Human, Occupational exposure, Occupational medicine, Poland, Europe, Biological monitoring, Biological marker, Industry, Blood plasma, ELISA assay
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0494549
Code Inist : 002B03L06. Création : 03/02/1998.