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  1. Copy number of the 16S rRNA gene in Coxiella burnetii.

    Article - En anglais

    Coxiella burnetii is an obligate intracellular bacterium with a doubling time of 5-7 hours.

    Chromosomal DNA from C. burnetii was digested with various restriction enzymes previously determined to not cut within the genomic 16S rRNA gene, or with a combination of these noncutting enzymes in conjunction with AflIII, a restriction enzyme that cuts twice within the 16S rRNA gene.

    Restriction fragments were resolved electrophoretically and probed with a radiolabeled DNA fragment containing the 3'AflIII portion of the C. burnetii 16S rRNA gene.

    Only a single DNA fragment in these digests hybridized to the probe, indicating that there is a single genomic copy of the 16S rRNA gene in C. burnetii and thus only a single copy of the rRNA operon.

    Mots-clés Pascal : Coxiella burnetii, Rickettsieae, Rickettsiaceae, Rickettsiales, Bactérie, Génotype, Gène, RNA ribosomique, Epidémiologie, Biologie moléculaire, Homme, Etats Unis, Amérique du Nord, Amérique

    Mots-clés Pascal anglais : Coxiella burnetii, Rickettsieae, Rickettsiaceae, Rickettsiales, Bacteria, Genotype, Gene, Ribosomal RNA, Epidemiology, Molecular biology, Human, United States, North America, America

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 97-0488991

    Code Inist : 002B05B02L8. Création : 03/02/1998.