The objective of this study was to evaluate the usefulness of nicotinamide adenine dinucleotide synthetase (NADS) activity for the biological monitoring of lead exposure.
The subjects were 76 male lead workers and 13 normal volunteers (7 males and 6 females).
NADS activity and blood lead concentration (Pb-B) was determined in each subject.
Delta-aminolevulinic acid dehydratase (ALAD) activity was determined in 58 lead workers out of 76 subjects.
NADS activity in the lead workers ranged from 0.08 to 1.1 mumol/h per g of hemoglobin (gHb) and decreased linearly (r=-0.867) as Pb-B increased up to 81.6 mug/dl.
The pattern of depressed activity of NADS was different from that of ALAD activity, which decreased rapidly and reached a plateau at the Pb-B level between 40 and 60 mug/dl.
The Pb-B levels inducing 50% inhibition of the enzyme activities were calculated to be 43 mug/dl and 20 mug/dl for NADS and ALAD, respectively.
At the Pb-B level of 40 mug/dl, NADS activity showed high validity (1.77) with predictivity of 0.92 at the cut-off level of 0.4 mumol/h per gHb, which were higher than those of ALAD activity.
These results show an apparent dose-effect relationship of NADS activity versus Pb-B.
NADS activity can be used for the biological monitoring of lead exposure.
Mots-clés Pascal : Plomb, Métal lourd, Toxicité, Surveillance biologique, Marqueur biologique, Exposition professionnelle, Médecine travail, Homme, Erythrocyte, Relation dose réponse, NAD+ synthase (glutamine-hydrolysing), Carbon-nitrogen ligases, Ligases, Enzyme
Mots-clés Pascal anglais : Lead, Heavy metal, Toxicity, Biological monitoring, Biological marker, Occupational exposure, Occupational medicine, Human, Red blood cell, Dose activity relation, NAD+ synthase (glutamine-hydrolysing), Carbon-nitrogen ligases, Ligases, Enzyme
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0476857
Code Inist : 002B03L05. Création : 03/02/1998.