Scientific Meeting of the Group of Chromatography and Related Techniques of the Spanish Royal Society of Chemistry. Barcelona (ESP), 1996/10/22.
A simplified method allowing the determination of trace concentrations (ng/ml) of hexachlorocyclohexanes in human sera suitable for the analysis of large numbers of samples has been developed.
Comparison of acid (conc.
H2SO4,) and basic (5 M KOH) digestion has shown that good recoveries are obtained with the former when an internal standard, 1,2,4,5-tetrabromobenzene (TBB), is used to correct for volatilization losses.
Good separation between alpha-bêta-and gamma-HCH isomers, TBB, hexachlorobenzene and some interfering compounds is obtained with DB-5 columns.
The use of electron-capture detection (ECD) or negative ion chemical ionization mass spectrometry (NICI-MS) in the selected ion monitoring mode (m/z 71) provides sufficient sensitivity for quantitative determination.
ECD is the most sensitive method for bêta-HCH and NICI-MS for the other isomers.
GC-NICI-MS is needed for the unambiguous determination of delta-HCH due to the coelution with one interfering compound.
The method has been successfully applied to the analysis of a series of 625 samples collected in a population situated near the effluents of an organochlorinated solvent factory.
The results have shown that bêta-HCH is selectively accumulated in human sera and prompt to the preferential investigation of the toxic effects of this isomer in humans and mammals.
Mots-clés Pascal : Analyse chimique, Analyse quantitative, Insecticide, Pesticide, Organochloré, HCH, Polluant, Liquide biologique, Sérum sanguin, Sang, Homme, Analyse trace, Préparation échantillon, Chromatographie phase gazeuse, Méthode couplée, Spectrométrie masse, Ionisation chimique, Détecteur capture électron, Surveillance biologique, Zone industrielle, Espagne, Europe
Mots-clés Pascal anglais : Chemical analysis, Quantitative analysis, Insecticide, Pesticides, Organochlorine compounds, HCH, Pollutant, Biological fluid, Blood serum, Blood, Human, Trace analysis, Sample preparation, Gas chromatography, Coupled method, Mass spectrometry, Chemical ionization, Electron capture detector, Biological monitoring, Industrial area, Spain, Europe
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0471920
Code Inist : 002B03G. Création : 03/02/1998.