Quantification of ergosterol and 3-hydroxy fatty acids in settled house dust by gas chromatography-mass spectrometry : Comparison with fungal culture and determination of endotoxin by a Limulus amebocyte lysate assay.
Ergosterol and 3-hydroxy fatty acids, chemical markers for fungal biomass and the endotoxin of gram-negative bacteria, respectively, may be useful in studies of health effects of organic dusts, including domestic house dust.
This paper reports a method for the combined determination of ergosterol and 3-hydroxy fatty acids in a single dust sample and a comparison of these chemical biomarkers determined by gas chromatography-mass spectrometry with results from fungal culture and Limulus assay.
Analyses of replicate house dust samples resulted in correlations of 0.91 (ergosterol in six replicates ; P<0.01) and 0.94 (3-hydroxy fatty acids in nine replicates ; P<0.001).
The amounts of ergosterol (range, 2 to 16.5 ng/mg of dust) correlated with those of total culturable fungi (range, 6 to 1,400 CFU/mg of dust) in 17 samples, (r=0.65 ; P<0.005).
The amounts of endotoxin (range, 11 to 243 endotoxin units/mg of dust) measured with a modified chromogenic Limulus assay correlated with those of lipopolysaccharide (LPS) determined from 3-hydroxy fatty acid analysis of 15 samples.
The correlation coefficient depended on the chain lengths of 3-hydroxy acids used to compute the LPS content.
The correlation was high (r=0.88 ± 0.01 ; P<0.001) when fatty acid chains of 10 to 14 carbon atoms were included ; the correlation was much lower when hydroxy acids of 16-or 18-carbon chains were included. (...)
Mots-clés Pascal : Poussière maison, Pollution intérieur, Acide gras, Origine microbienne, Fungi, Thallophyta, Bactérie, Culture microorganisme, Analyse quantitative, Test Limulus, Chromatographie phase gazeuse, Endotoxine, Spectrométrie masse, Allergène, Etude comparative, Ergocalciférol
Mots-clés Pascal anglais : House dust, Indoor pollution, Fatty acids, Microbial origin, Fungi, Thallophyta, Bacteria, Microorganism culture, Quantitative analysis, Limulus lysate assay, Gas chromatography, Endotoxin, Mass spectrometry, Allergen, Comparative study, Ergocalciferol
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0411600
Code Inist : 001D16C06. Création : 19/12/1997.