To estimate the prevalence of Chlamydia trachomatis in asymptomatic women attending general practice ; to assess the potential of the ligase chain reaction as a screening tool ; and to evaluate selective screening criteria.
Cross sectional survey.
Four general practices in northeast London.
890 women aged 18-35 years attending general practice for a cervical smear or a « young well woman » check between October 1994 and January 1996.
The women were tested for C trachomatis with confirmed enzyme immunoassay (endocervical specimens) and ligase chain reaction assay on urine specimens.
Prevalence of C trachomatis infection in women aged 18-35 on the basis of each test ; sensitivity and specificity of both tests in this population.
Prevalence of confirmed infection was 2.6% (95% confidence interval 1.6% to 3.6%) in all women.
Prevalence on the basis of enzyme immunoassay was 1.6% (0.8% to 2.7%), with a sensitivity of 60% and a specificity of 100%. Prevalence on the basis of ligase chain reaction was 2.5% (1.5%) to 3.9%), with 90% sensitivity and 99.8% specificity.
Screening all women aged ¾25 and all women who had had two or more partners in the past year would have detected 87% (20/23) of infections.
Ligase chain reaction on urine samples performs at least as well as enzyme immunoassay on cervical specimens in this low prevalence population. (...)
Mots-clés Pascal : Maladie sexuellement transmissible, Chlamydia trachomatis, Chlamydiaceae, Chlamydiales, Bactérie, Prévalence, Ligases, Enzyme, Réaction chaîne polymérase, Etude transversale, Méthode immunoenzymatique, Etude comparative, Femelle, Homme, Evaluation, Médecin généraliste, Appareil génital pathologie, Education santé, Activité biologique
Mots-clés Pascal anglais : Sexually transmitted disease, Chlamydia trachomatis, Chlamydiaceae, Chlamydiales, Bacteria, Prevalence, Ligases, Enzyme, Polymerase chain reaction, Cross sectional study, Enzyme immunoassay, Comparative study, Female, Human, Evaluation, General practitioner, Genital diseases, Health education, Biological activity
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0396607
Code Inist : 002B05F06. Création : 12/09/1997.