Airway isocyanate-adducts in asthma induced by exposure to hexamethylene diisocyanate.
Objectives The clinical features, airway histology, and detection of hexamethylene diisocyanate (HDI) protein adducts in endobronchial biopsies from a patient with HDI asthma are described.
Methods Isocyanate asthma was diagnosed by history, methacholine challenge, and workplace HDI challenge.
Bronchoscopy was performed 24 h after challenge and immunohistochemical staining was performed.
Results Airway biopsies obtained at bronchoscopy demonstrated inflammatory changes typical for asthma, including increased airway eosinophils and T cells.
Immunohistochemical staining with specific anti-HDI antibodies demonstrated the presence and localization of HDI adducts in human bronchial biopsies.
Conclusions These studies confirm epithelial exposure to HDI following workplace challenge and demonstrate the feasibility of detecting and localizing isocyanate adducts in human lung tissue.
Identifying and characterizing the airway macromolecules to which isocyanates bind in vivo are probably crucial to the understanding of how isocyanates cause sensitization and asthma.
The ability to detect isocyanate adducts may also help characterize isocyanate exposure patterns and exposure-disease relationships.
Mots-clés Pascal : Diisocyanate organique, Toxicité, Symptomatologie, Asthme, Homme, Exposition professionnelle, Médecine travail, Voie respiratoire, Adduit moléculaire, Marqueur biologique, Diagnostic, Appareil respiratoire pathologie, Bronchopneumopathie obstructive, Hexane(1,6-diisocyanato)
Mots-clés Pascal anglais : Organic diisocyanate, Toxicity, Symptomatology, Asthma, Human, Occupational exposure, Occupational medicine, Respiratory tract, Molecular adduct, Biological marker, Diagnosis, Respiratory disease, Obstructive pulmonary disease
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0391597
Code Inist : 002B03L06. Création : 12/09/1997.