Oral diagnostic testing for detecting human immunodeficiency virus-1 antibodies : A technology whose time has come.
An oral fluid-based test for antibodies to human immunodeficiency virus (HIV), equivalent to serum in its accuracy but safer and easier to use, is now available in the United States.
The development of the oral test involved overcoming technical obstacles to the use of oral fluid as a testing medium, including low immunoglobulin G (IgG) titers, suboptimal assay performance, protease degradation of IgG, high viscosity, and lack of a standardized method of specimen collection, all of which contribute to suboptimal assay performance.
The currently available oral HIV test utilizes a collection device to isolate a mucosal transudate component of oral fluid rich in IgG.
A vial containing a preservative solution facilitates the transport of stable, low-viscosity specimens to the laboratory for testing with an ELISA and confirmatory Western blot assay, specifically designed for use with oral fluid.
Non-HIV medical conditions and oral pathologies do not appear to affect oral test results.
Hopefully, the availability of a more patient-friendly, portable diagnostic test for antibodies to HIV will facilitate identification of greater numbers of infected individuals with the ultimate goals of early identification, early treatment, and prevention of disease transmission.
Mots-clés Pascal : SIDA, Virose, Infection, Virus immunodéficience humaine, Lentivirinae, Retroviridae, Virus, Diagnostic, Test absorption, Forme orale, Etude comparative, Forme fluide, Evaluation, Etude expérimentale, Prévention, Homme, Etats Unis, Amérique du Nord, Amérique, Immunopathologie, Immunodéficit, Organisation santé, Activité biologique, Voie abord
Mots-clés Pascal anglais : AIDS, Viral disease, Infection, Human immunodeficiency virus, Lentivirinae, Retroviridae, Virus, Diagnosis, Absorption test, Oral form, Comparative study, Fluid form, Evaluation, Experimental study, Prevention, Human, United States, North America, America, Immunopathology, Immune deficiency, Public health organization, Biological activity, Surgical approach
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0361194
Code Inist : 002B06D01. Création : 12/09/1997.