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  1. Pulmonary expression of vascular endothelial growth factor and myosin isoforms in rats with congenital diaphragmatic hernia.

    Article, Communication - En anglais

    Annual International Congress of the British Association of Paediatric Surgeons. St Helier, Jersey (GBR), 1996/07/16.

    Abnormalities of the pulmonary vasculature are well documented in cases of congenital diaphragmatic hernia (CDH).

    Vascular endothelial growth factor (VEGF), an angiogenic factor, is a recently described endothelial cell-specific growth factor.

    Myosin heavy chain (MHC) isoforms such as SMemb, SM1 and SM2 are important molecular markers used to study vascular smooth muscle cell differentiation.

    SMemb is predominantly expressed in immature smooth muscle cells (SMC), and SM2 is expressed in mature SMCs.

    The authors investigated the expression of VEGF and SMC differentiation in pulmonary vessels in CDH rat lungs and in controls.

    The lungs of nitrofen-induced CDH rat fetuses (n=16, gestational age 16,18,20, and 22 days) were stained immunohistochemically using antibodies against VEGF, SMemb and SM2, while alpha-actin was used as a general marker of vascular smooth muscle cells.

    In the CDH group VEGF expression was negative in pulmonary vessels before birth, and in the control group VEGF was positive in smooth muscle cells in vessel walls from 20 days both in vessels at the hilum and in pulmonary parenchyma.

    In both control and CDH groups, SMemb expression was positive from 16 days'gestation.

    SM2 expression was negative in vessel walls during the prenatal period in both groups.

    Alpha-actin was localized in both lungs obtained from control and CDH groups in the lung hilum from 16 days and around peripheral vessels from 18 days. (...)

    Mots-clés Pascal : Hernie, Diaphragme, Congénital, Oscillateur haute fréquence, Ventilation, Etude comparative, Facteur prédictif, Pédiatrie, Protocole thérapeutique, Circuit extracorporel, Oxygénateur membrane, Evaluation, Traitement, Muscle respiratoire, Appareil respiratoire pathologie, Malformation, Traitement instrumental, Appareil circulatoire pathologie

    Mots-clés Pascal anglais : Hernia, Diaphragm, Congenital, High frequency oscillator, Ventilation, Comparative study, Predictive factor, Pediatrics, Therapeutic protocol, Extracorporeal circuit, Membrane oxygenator, Evaluation, Treatment, Respiratory muscle, Respiratory disease, Malformation, Instrumentation therapy, Cardiovascular disease

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    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 97-0360489

    Code Inist : 002B11D. Création : 12/09/1997.