Background Laboratory animal workers are at high risk of developing occupational allergy.
Little is known about the relationship between levels of exposure and the risk of developing laboratory animal allergy.
Since laboratory animal work comprises a large number of different - often short lasting - tasks, it is of interest to assess which activities are associated with high, low or intermediate levels of allergen exposure.
Objective To develop and evaluate highly sensitive immunoassays in order to quantify rat and mouse urinary allergens in airborne dust sampled during short-lasting tasks.
Methods Personal air dust samples were taken during full-shift periods as well as during specific tasks in seven laboratory animal facilities.
Two sandwich enzyme immunoassays were developed, using rabbit antisera against rat and mouse urinary proteins.
The rabbit antibodies were analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting and compared with IgE antibodies from sensitized laboratory animal workers.
Results The rabbit antibodies were highly specific for rat and mouse urinary proteins and reacted with all IgE-binding allergens in either urinary protein preparation.
The assays for rat and mouse urine were very sensitive, with detection limits of 0.075ng/mL.
The coefficient of variation of the analysis was 12.9% for both assays.
Animal caretakers appeared to experience the highest exposure to aeroallergens. (...)
Mots-clés Pascal : Allergie, Exposition professionnelle, Personnel laboratoire, Animal laboratoire, Souris, Rodentia, Mammalia, Vertebrata, Rat, Allergène, Origine animale, Analyse quantitative, Teneur air ambiant, Analyse risque, Méthode immunoenzymatique, Poste travail, Activité professionnelle, Homme, Immunopathologie, Médecine travail
Mots-clés Pascal anglais : Allergy, Occupational exposure, Laboratory staff, Laboratory animal, Mouse, Rodentia, Mammalia, Vertebrata, Rat, Allergen, Animal origin, Quantitative analysis, Ambient air concentration, Risk analysis, Enzyme immunoassay, Workplace layout, Professional activity, Human, Immunopathology, Occupational medicine
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0340727
Code Inist : 002B06C05. Création : 12/09/1997.