An assay combining cell culture with reverse transcriptase PCR to detect and determine the infectivity of waterborne Cryptosporidium parvum.
The presence of Cryptosporidium in drinking water supplies is a significant problem faced by the water industry.
Although a variety of methods exist for the detection of waterborne oocysts, water utilities currently have no way of assessing the infectivity of detected oocysts and consequently are unable to accurately determine the risks posed to public health by waterborne Cryptosporidium.
In this paper, the development of an infectivity assay for waterborne Cryptosporidium parvum is described.
Oocysts were inoculated onto monolayers of Caco-2 cells and grown on microscope slides, and infections were detected by C. parvum specific reverse transcriptase PCR of extracted mRNA, targeting the heat shock protein 70 (hsp70) gene.
A single infectious oocyst was detected by this experimental procedure.
The use of concentrated samples obtained from 250 liters of finished water had no observable effect on the integrity of cell monolayers or on the infectivity of oocysts seeded into the concentrate.
Intracellular developmental stages of the parasite were also detected by using fluorescently labeled antibodies.
One pair of PCR primers targeting the hsp70 gene was specific for C. parvum, while a second pair recognized all species of Cryptosporidium tested.
The C. parvum-specific primers amplified DNA from 1 to 10 oocysts used to seed 65 to 100 liters of concentrated environmental water samples and were compatible with multiplex PCR for the simultaneous detection of C. (...)
Mots-clés Pascal : Cryptosporidium parvum, Sporozoa, Protozoa, Pathogène, Pouvoir infectant, Culture cellulaire, In vitro, Transcription inverse, Réaction chaîne polymérase, Détection, Méthode, Parasite, Homme, Côlon, Lignée cellulaire, Lignée CACO2, Gène hsp70
Mots-clés Pascal anglais : Cryptosporidium parvum, Sporozoa, Protozoa, Pathogenic, Infectivity, Cell culture, In vitro, Reverse transcription, Polymerase chain reaction, Detection, Method, Parasite, Human, Colon, Cell line
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0318332
Code Inist : 002A11A. Création : 12/09/1997.