Chronic suppurative otitis media, averaging 20 or more years of duration, has been associated with cancer in this region in 40% - 80% of cases.
Although human papillomaviruses (HPV) have been implicated in many human squamous-cell neoplasms, their role in the pathogenesis of middle-ear malignancies remains unexplored.
In this study, we investigated the presence and subtypes of HPV in middle-ear carcinomas.
Formalin-fixed and paraffin-embedded tumor tissues were sampled for DNA extraction.
PCR was done with consensus primers, capable of detecting HPV 16,18,31,33,52b and 58.
Typing of the products generated by consensus primers was performed with restriction enzyme digestion.
It was found that a resulting 89% (8/9) of the middle-ear carcinomas contained HPV DNA.
Coexistence of HPV 16 and 18 was detected in 3 squamous-cell carcinomas.
HPV 16 was detected in 4 squamous-cell carcinomas and I adenocarcinoma.
The high prevalence of high-risk-type HPV in carcinomas of the middle ear suggests that viral infection may be an important etiologic component in the carcinogenic process.
Mots-clés Pascal : Carcinome, Oreille moyenne, Homme, Papillomavirus humain 16, Papillomavirus, Papovaviridae, Virus, Papillomavirus humain 18, Association, Réaction chaîne polymérase, DNA, Exploration, Carcinogenèse, Pathogénie, Epidémiologie, Tumeur maligne, ORL pathologie, Oreille moyenne pathologie, Biologie moléculaire
Mots-clés Pascal anglais : Carcinoma, Middle ear, Human, Human papillomavirus 16, Papillomavirus, Papovaviridae, Virus, Human papillomavirus 18, Association, Polymerase chain reaction, DNA, Exploration, Carcinogenesis, Pathogenesis, Epidemiology, Malignant tumor, ENT disease, Middle ear disease, Molecular biology
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0285992
Code Inist : 002B10D01. Création : 15/07/1997.