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  1. Quantitative colorimetric determination of urinary p-aminophenol with an automated analyzer.

    Article - En anglais

    We developed an automated colorimetric method for the quantitative determination of p-aminophenol with a Cobas Mira analyzer.

    The procedure can be used for the biological monitoring of human exposure to aniline.

    An absorbed aniline dose is extensively oxidized to p-aminophenol, which is excreted in urine mainly as glucurono-and sulfo-conjugates.

    After enzymatic hydrolysis, we reacted the free compound with resorcinol in the presence of manganese ions to form an indophenol dye, which is measured at 550 nm.

    Excellent accuracy (102.8%, 103.9%, and 96.8% at 2.5,50, and 90 mg/L, respectively) and precision (7.7%, 2.1%, and 0.8% CV for within-run and 11.1%, 4.7%, and 4.6% for total reproducibility at 2.5,50, and 90 mg/L, respectively) were achieved over a linear concentration range of 2.0 to 100 mg/L. The detection limit was 0.9 mg/L and no significant interference (except for o-aminophenol) was found for several investigated drugs and related compounds.

    The proposed method was used for a stability study and to analyze several samples from an occupational health screen.

    Mots-clés Pascal : Aniline, Exposition professionnelle, Méthode étude, Marqueur biologique, Aminophénols, Urine, Homme, Analyse quantitative, Colorimétrie, Automatisation, Médecine travail, Surveillance biologique, Liquide biologique

    Mots-clés Pascal anglais : Aniline, Occupational exposure, Investigation method, Biological marker, Aminophenols, Urine, Human, Quantitative analysis, Colorimetry, Automation, Occupational medicine, Biological monitoring, Biological fluid

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 97-0249851

    Code Inist : 002B03L06. Création : 11/06/1997.