As part of a prospective study to evaluate maternal and neonatal scalp hair mineral profiles in normal and pathological pregnancy cases, the suitability of inductively coupled plasma-mass spectrometry (ICP-MS) was evaluated for the quantitation of mercury and selenium as part of a multi-element determination procedure.
Treatment of hair samples included a closed-system microwave-assisted digestion in screw-capped PTFE vials of 10-mL capacity with concentrated nitric acid (2 mL) as the matrix solubilization medium.
A digestion time of 10 min at 40% microwave power yielded limpid digestates, which, after appropriate dilution with deionized water to give a final acid concentration of 20% (v/v), were used for direct ICP-MS measurements within a rectilinear calibration range from 0 to 10 mug/L. Analytical recovery results for mercury ranged from 97 to 102%, whereas those for selenium ranged from 96 to 101%. Results from concurrent analyses of a human hair reference material showed a high degree of concordance with certified values.
The results obtained suggest that the closed-system microwave-assisted digestion procedure described was effective in minimizing the risk of volatility-associated losses that can be encountered in the assays of both metals.
The results also indicated that the ICP-MS system used in this study was suitable for including both metals as part of a multi-element quantitation procedure. (...)
Mots-clés Pascal : Analyse chimique, Analyse quantitative, Mercure, Métal lourd, Sélénium, Oligoélément, Elément minéral, Mesure simultanée, Analyse multiélément, Matériel biologique, Cheveu, Femme, Homme, Gestation, Nouveau né, Surveillance biologique, Royaume Uni, Europe, Spectrométrie ICP, Méthode couplée, Spectrométrie masse
Mots-clés Pascal anglais : Chemical analysis, Quantitative analysis, Mercury, Heavy metal, Selenium, Trace element (nutrient), Inorganic element, Simultaneous measurement, Multicomponent analysis, Biological material, Hair (head), Woman, Human, Pregnancy, Newborn, Biological monitoring, United Kingdom, Europe, Inductive coupling plasma spectrometry, Coupled method, Mass spectrometry
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0228559
Code Inist : 002B03L05. Création : 11/06/1997.