Objectives This study investigated the possibility of applying serum pantropic p53 proteins in molecular epidemiologic studies, as a biomarker of environmental carcinogenesis.
The serum levels of pantropic p53 proteins were determined in workers with past exposure to hexavalent chromium compounds.
Methods Thirty-one male workers occupationally exposed to hexavalent chromium compounds in the production of chromium compounds for 0 to 23 years served as the exposed group.
The referents were 10 volunteers without work-related exposure to chemicals.
In the determination of pantropic p53 proteins, commercially available kits for enzyme-linked immunosorbent assay were used which quantitatively detected both mutant and wild-type human p53 proteins.
Results The serum level of pantropic p53 proteins was in the range of 116.4 to 1 122.6 pg/ml for the exposed workers and of 117.4 to 305.8 pg/ml for the referents.
Nineteen percent of the exposed workers had a high p53 protein level (6 out of 31) when compared with the referents.
All but I of the 6 workers had been occupationally exposed to chromium compounds for more than 11 years.
Two of the 3 workers with a past history of lung cancer also showed high levels.
Conclusions Our findings confirm that the application of p53 proteins as a biomarker of environmental carcinogenesis merits further exploration.
Mots-clés Pascal : Chrome VI, Métal lourd, Toxicité, Exposition professionnelle, Médecine travail, Homme, Antécédent, Marqueur biologique, Epidémiologie moléculaire, Surveillance biologique, Sérum, Liquide biologique, Protéine p53, Histoire professionnelle
Mots-clés Pascal anglais : Chromium VI, Heavy metal, Toxicity, Occupational exposure, Occupational medicine, Human, Antecedent, Biological marker, Molecular epidemiology, Biological monitoring, Serum, Biological fluid
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0228235
Code Inist : 002B03L05. Création : 11/06/1997.