HCV is ubiquitous.
In 50% of all cases it causes chronic hepatitis that often evolves into liver cirrhosis and hepatocellular carcinoma.
Recently HCV has been classified in 5 genotypes by Okamoto.
The purpose of this study is to evaluate the prevalence of 5 genotypes in Campania, a region of southern Italy, where the prevalence of anti-HCV antibodies ranges from 0.87 to 4%, and to evaluate the correlation between the HCV genotypes and the severity of histological damage.
One-hundred-and-thirty-five anti-HCV positive patients were enrolled and tested by PCR to identify HCV-RNA.
One-hundred-and-twenty-four patients resulted HCV-RNA positive.
Genotyping was performed as described by Okamoto et al. with minor modifications of the specific primer to type III proposed by Silini et al.
Eight patients were negative for all genotypes.
Eight patients were positive for type I (la), 61 for type II (lb), 39 for type III (2a), 11 for type IV (2b) and 1 for type V (3a).
In 4 cases two different genotypes were present in the same sample [II (1b) - IV (2b), III (2a) - II (1b) twice, III (2a) - IV (2b) ]. Histological evaluation of liver damage showed : CPH (22 cases), minimal CAH (56), severe CAH (31) and liver cirrhosis (15).
There was no statistically significant correlation between the 5 genotypes and the severity of histological damage.
Data on the prevalence of genotype II (lb) in Italy are similar to those reported for other European countries. (...)
Mots-clés Pascal : Hépatite virale C, Virose, Infection, Génotype, Virus hépatite C, Flaviviridae, Virus, Répartition géographique, Réaction chaîne polymérase, Epidémiologie, Prévalence, Facteur risque, Indice gravité, Homme, Italie, Europe, Appareil digestif pathologie, Foie pathologie, Biologie moléculaire
Mots-clés Pascal anglais : Viral hepatitis C, Viral disease, Infection, Genotype, Hepatitis C virus, Flaviviridae, Virus, Geographic distribution, Polymerase chain reaction, Epidemiology, Prevalence, Risk factor, Severity score, Human, Italy, Europe, Digestive diseases, Hepatic disease, Molecular biology
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0227506
Code Inist : 002B05C02G. Création : 11/06/1997.