Differentiation between cosensitization and cross-reactivity in wheat flour and grass pollen-sensitized subjects.
To diagnose baker's asthma. occupational sensitization to wheat flour should be distinguished clearly from influences ofcosensitization such as it may exist in pollinosis patients.
To define the route of sensitization. the cross-reactivity between wheat flour and grass pollen allergens was investigated.
Two groups ofatopic individuals with or without professional contact to wheat flour were screened by skin prick test for their sensitization to wheat flour and grass pollen and, in the case of hints for cosensitization, by Enzyme Allergo Sorbent Test (EAST).
Cross-reactivity between wheat flour and grass pollen allergens was investigated by IgE binding inhibition assay using sera of 20 cosensitized individuals and by immunoblots.
The immunological cross-inhibition between wheat flour and grass pollen proves some proteins to share common allergenic determinants.
Significant differences between bakers and individuals not occupationally exposed to flour could be seen in the inhibition of IgE binding to wheat flour.
While the IgE binding to wheat flour allergens was only slightly inhibited by grass pollen proteins in bakers sera its inhibition was nearly complete by this extract in sera of nonexposed atopics.
Immunoblots indicate that wheat proteins with a molecular weight ofabout 8-12 and 22 kD preferentially react with sensitized bakers'IgE and not with IgE of other individuals. (...)
Mots-clés Pascal : Asthme, Boulanger, Allergie, Atopie, Farine blé, Pollen, Allergène, Gramineae, Monocotyledones, Angiospermae, Spermatophyta, Réaction croisée, Etude comparative, Exposition professionnelle, Homme, Appareil respiratoire pathologie, Bronchopneumopathie obstructive, Immunopathologie, Maladie professionnelle, Médecine travail
Mots-clés Pascal anglais : Asthma, Baker, Allergy, Atopy, Wheat flour, Pollen, Allergen, Gramineae, Monocotyledones, Angiospermae, Spermatophyta, Cross reaction, Comparative study, Occupational exposure, Human, Respiratory disease, Obstructive pulmonary disease, Immunopathology, Occupational disease, Occupational medicine
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0217665
Code Inist : 002B06C02. Création : 21/05/1997.