A multicentric study was conducted to evaluate the frequency of Mercurialis annua pollen sensitization in several areas of Spain and to select a population sample to characterize the main allergenic components in M. annua pollen.
Patients were recruited from six hospitals in Spain.
Out of 420 patients sensitized to pollens.
195 46.4% showed positive skin tests to M. annua, thus evidencing the high level of sensitization to the pollen of this plant in Spain.
Thirty-seen sera with RAST class values to M. annua =3 were selected for SDS-PAGE immunoblotting analysis.
Two main allergenic components with molecular weigths of 15.8 and 14.1 kD were detected in 59 and 51% of the sera. respectively and they were identified as profilins.
Isolation ofthe relevant allergens was made by affinity. chromatography on a poly-L-proline - Sepharose column. followed by gel filtration and anion exchange chromatography in the micropreparative SMART System.
A significant but low antigenic cross-reactiitj, between, if. iinnia and Olea europaea, Fraxinus elatior, Ricinus communis, Salsola Kali, Parietaria judaica and Artemisia vulgaris was demonstrated by several in vitro techniques.
Mots-clés Pascal : Allergie, Pollen, Mercurialis, Euphorbiaceae, Dicotyledones, Angiospermae, Spermatophyta, Prévalence, Epidémiologie, Allergène, Réaction croisée, Olea europaea, Oleaceae, Arbre, Fraxinus, Ricinus communis, Artemisia, Compositae, Espagne, Europe, Homme, Immunopathologie, Urticaceae, Mercurialis annua, Parietaria judaica, Salsola kali, Profiline
Mots-clés Pascal anglais : Allergy, Pollen, Mercurialis, Euphorbiaceae, Dicotyledones, Angiospermae, Spermatophyta, Prevalence, Epidemiology, Allergen, Cross reaction, Olea europaea, Oleaceae, Tree, Fraxinus, Ricinus communis, Artemisia, Compositae, Spain, Europe, Human, Immunopathology, Urticaceae
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0217661
Code Inist : 002B06C01. Création : 21/05/1997.