DNA single strand breakage, DNA adducts, and sister chromatid exchange in lymphocytes and phenanthrene and pyrene metabolites in urine of coke oven workers.
Objectives-To investigate the specificity of biological monitoring variables (excretion of phenanthrene and pyrene metabolites in urine) and the usefulness of some biomarkers of effect (alkaline filter elution, 32P postlabelling assay, measurement of sister chromatid exchange) in workers exposed to polycyclic aromatic hydrocarbons (PAHs).
Methods-29 coke oven workers and a standardised control group were investigated for frequencies of DNA single strand breakage, DNA protein cross links (alkaline filter elution assay), sister chromatid exchange, and DNA adducts (32P postlabelling assay) in lymphocytes.
Phenanthrene and pyrene metabolites were measured in 24 hour urine samples. 19 different PAHs (including benzo- (a) pyrene, pyrene, and phenanthrene) were measured at the workplace by personal air monitoring.
The GSTT1 activity in erythrocytes and lymphocyte subpopulations in blood was also measured.
Concentrations of phenanthrene, pyrene, and benzo (a) pyrene in air correlated well with the concentration of total PAHs in air ; they could be used for comparisons of different workplaces if the emission compositions were known.
The measurement of phenanthrene metabolites in urine proved to be a better biological monitoring variable than the measurement of 1-hydroxypyrene. (...)
Mots-clés Pascal : Four coke, Phénanthrène, Pyrène, Composé aromatique polycyclique, Toxicité, Exposition professionnelle, Médecine travail, Homme, Surveillance biologique, Cassure chromosomique, Adduit moléculaire, DNA, Métabolite, Urine, Marqueur biologique, Liquide biologique, Lymphocyte
Mots-clés Pascal anglais : Coke oven, Phenanthrene, Pyrene, Polycyclic aromatic compound, Toxicity, Occupational exposure, Occupational medicine, Human, Biological monitoring, Chromosome break, Molecular adduct, DNA, Metabolite, Urine, Biological marker, Biological fluid, Lymphocyte
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0209242
Code Inist : 002B03L06. Création : 21/05/1997.