Determination of atrazine levels in whole saliva and plasma in rats : Potential of salivary monitoring for occupational exposure.
Current biological monitoring techniques are often unable to provide accurate estimates of pesticide dose in exposed worker populations.
This study was conducted to investigate the feasibility of pesticide biomonitoring using saliva.
Atrazine [2-chloro-4-ethylamino-6- (isopropylamino) - s-triazine], a member of the triazine herbicides, was selected to investigate salivary excretion following direct gastric administration in rats.
Concentrations of atrazine in whole saliva and arterial plasma samples were determined by enzyme-linked immunosorbent assay (ELISA).
Atrazine reached its highest level in both arterial plasma (238 mug/L) and whole saliva (157 mug/L) 35 min after administration of 105 mg/kg of atrazine, and then decreased with time in a parallel fashion.
Although saliva atrazine levels were lower than levels in arterial plasma, there was a very high correlation between whole saliva and arterial plasma atrazine concentrations (gamma2=95).
In addition, pharmacokinetic analysis suggested that salivary levels of atrazine can be used to predict concentrations of atrazine in plasma.
The mean whole saliva/arterial plasma atrazine concentration ratio (S/P) was 0.66 ± 0.11 (n=20).
The S/P ratios did not vary significantly over time, and were not affected by salivary flow rate.
This study demonstrates that atrazine is transported into saliva, and that a relatively constant concentration ratio between whole saliva and arterial plasma is maintained. (...)
Mots-clés Pascal : Surveillance biologique, Salive, Liquide biologique, Exposition professionnelle, Médecine travail, Toxicocinétique, Cinétique, Rat, Rodentia, Mammalia, Vertebrata, Animal, Plasma sanguin, Sang, Pesticide, Herbicide, Atrazine, Triazine dérivé
Mots-clés Pascal anglais : Biological monitoring, Saliva, Biological fluid, Occupational exposure, Occupational medicine, Toxicokinetics, Kinetics, Rat, Rodentia, Mammalia, Vertebrata, Animal, Blood plasma, Blood, Pesticides, Herbicide, Atrazine, Triazine derivatives
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0207440
Code Inist : 002B02G. Création : 21/05/1997.