Ninety-seven strains, producing a fluorescent pigment under UV light and/or a green diffusive pigment on cetrimide-naladixic acid agar, were isolated from a spring water bottling plant.
These strains were presumptively identified as Pseudomonas aeruginosa, but they could not be confirmed as strains of this species nor identified by the API 20NE identification system.
The isolates and reference strains were clustered by computer-assisted whole-cell protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
The numerical analysis of the protein electrophoregrams resulted in the formation of four clusters at a similarity level of 80% and two unclustered type strains.
One cluster included strains isolated during a 4-month period and reference strains of several biotypes of P. fluorescens.
The remaining isolates formed another cluster with a very high similarity level, which included two groups of strains based on biochemical characterization by the API 20NE Test System.
Strains were typed by random amplified polymorphic DNA (RAPD) - PCR and two different RAPI) patterns were obtained, corresponding to each biochemical profile.
This persistent colonization seems to be caused by a single species present in the bottling system, with two clonal origins, not related to P. aeruginosa or to any of the other type strains tested.
Partial 16S rDNA sequence of a representative strain of one cluster of isolates had a level of similarity of 99.3% with P. alcaligenes. (...)
Mots-clés Pascal : Usine, Embouteillage, Contamination biologique, Pseudomonas, Pseudomonadaceae, Pseudomonadales, Bactérie, Pathogène, Isolat, DNA ribosomique, Caractérisation, RNA 16S, Typage, Séquence nucléotide, Biotype, Homologie, Marqueur RAPD, Qualité eau, Marqueur génétique, Colonisation, Eau source
Mots-clés Pascal anglais : Industrial plant, Bottling, Biological contamination, Pseudomonas, Pseudomonadaceae, Pseudomonadales, Bacteria, Pathogenic, Isolate, Ribosomal DNA, Characterization, 16S-RNA, Typing, Nucleotide sequence, Biotype, Homology, Random amplified polymorphic DNA, Water quality, Genetic marker, Colonization
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0197683
Code Inist : 002A35B17. Création : 21/05/1997.