Comparison of a monoclonal antibody-based IgM capture ELISA with a neutralization assay for assessing response to trivalent oral poliovirus vaccine.
Monoclone-based IgM capture ELISAs were developed for each of the three poliovirus serotypes and compared with a neutralization assay for detecting response to trivalent oral poliovirus vaccine among 224 infants.
The IgM-based response rates were significantly higher than the neutralizing antibody-based rates : 95% versus 83% to poliovirus type 1,99% versus 94% to poliovirus type 2, and 89% versus 59% to poliovirus type 3. IgM responses to the first vaccine dose were significantly associated between serotypes, suggesting that some of the discordance may reflect a heterotypic IgM response.
When the response rates in 4 vaccine formulation groups were compared, group differences using the two assays were similar for poliovirus types 1 and 2 but not for type 3. Therefore, IgM results using these assays may not be adequate substitutes for neutralizing antibody results when determining vaccine response.
Mots-clés Pascal : Poliomyélite antérieure, Virose, Infection, Poliovirus, Enterovirus, Picornaviridae, Virus, Immunoprophylaxie, Vaccination, Vaccin polyvalent, Voie orale, Evaluation, Efficacité, Anticorps monoclonal, Technique ELISA, IgM, Test neutralisation, Etude comparative, Homme, Système nerveux pathologie, Système nerveux central pathologie, Moelle épinière pathologie, Génie biomédical
Mots-clés Pascal anglais : Acute anterior poliomyelitis, Viral disease, Infection, Poliovirus, Enterovirus, Picornaviridae, Virus, Immunoprophylaxis, Vaccination, Polyvalent vaccine, Oral administration, Evaluation, Efficiency, Monoclonal antibody, ELISA assay, IgM, Neutralization test, Comparative study, Human, Nervous system diseases, Central nervous system disease, Spinal cord disease, Biomedical engineering
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0188147
Code Inist : 002B05A02. Création : 21/05/1997.