Detection of snow-crab antigens by air sampling of a snow-crab production plant.
Background and objective We previously assessed the prevalence of occupational asthma (OA) to snow-crab in production plant workers.
We also showed that this type of OA is related to immediate immunological reactivity as demonstrated by skin reactivity and increased specific IgE antibodies.
However, we did not show that snow-crab antigens causing immunological reactivity and OA could be found in the air sampling in the plants atmosphere.
This was the purpose of the current work.
Material and methods Area air samples worn by workers at four different worksites of a snow-crab producing plant were obtained on PVC filters with an SKC pump run at 1.5 L/min for 2 h. Snow-crab was being boiled and processed during the air sampling periods.
Filters were analysed by RAST inhibition in a blind manner (i.e. without knowledge of the worksite where the filter originated).
Results Eluate from one of the four sites (2) had the highest protein concentration and yielded the highest per cent inhibition of RAST 13% inhibition with the snow-crab meat, 23% and 28% inhibition with the snow crab water RAST in two separate assays.
An eluate taken from a filter at another site (1) showed borderline reactivity (1% and 10% inhibition in two assays) whereas the two other ones and a control filter were negative.
The two filters that contained snow-crab proteins were the ones nearest the boiling process, site 2 being the nearest followed by site 1. (...)
Mots-clés Pascal : Asthme, Aliment, Allergie, Crabe, Brachyura, Decapoda, Crustacea, Arthropoda, Invertebrata, Exposition professionnelle, Etiologie, Canada, Amérique du Nord, Amérique, Homme, Appareil respiratoire pathologie, Bronchopneumopathie obstructive, Maladie professionnelle, Médecine travail, Immunopathologie, Pneumallergène
Mots-clés Pascal anglais : Asthma, Food, Allergy, Crab, Brachyura, Decapoda, Crustacea, Arthropoda, Invertebrata, Occupational exposure, Etiology, Canada, North America, America, Human, Respiratory disease, Obstructive pulmonary disease, Occupational disease, Occupational medicine, Immunopathology, Aeroallergen
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0167911
Code Inist : 002B06C02. Création : 21/05/1997.