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  1. Bacteria, molds, and toxins in water-damaged building materials.

    Article - En anglais

    Microbial toxins and eukaryotic cell toxicity from indoor building materials heavily colonized by fungi and bacteria were analyzed.

    The dominant colonizers at water-damaged sites of the building were Stachybotrys chartarum (103 to 105 visible conidia cm-2), Penicillium and Aspergillus species (104 CFU mg-1), gram-negative bacteria (104 CFU mg-1), and mycobacteria (103CFU mg-1).

    The mycobacterial isolates were most similar to M. komossense, with 98% similarity of the complete 16S rDNA sequence.

    Limulus assay of water extracts prepared from a water-damaged gypsum liner revealed high contents of gram-negative endotoxin (17 ng mg-1 of E. coli lipopolysaccharide equivalents) and bêta-D-glucan (210 ng mg-1of curdlan equivalents).

    High-performance liquid chromatography analysis of the methanol extracts showed that the water-damaged gypsum liner also contained satratoxin (17 ng mg-1).

    This methanol-extracted substance was 200 times more toxic to rabbit skin and fetus feline lung cells than extract of gypsum liner sampled from a non-water-damaged site.

    The same extract contained toxin (s) that paralyzed the motility of boar spermatozoa at extremely low concentrations ; the 50% effective concentration was 0.3 mug of dry solids per ml.

    This toxicity was not explainable by the amount of bacterial endotoxin, bêta-D-glucan, or satratoxin present in the same extract. (...)

    Mots-clés Pascal : Endotoxine, Test cytotoxicité, Aspergillus, Fungi Imperfecti, Fungi, Thallophyta, Penicillium, Bâtiment, Matériau construction, Toxine, Détection, Caractérisation, Pathogène, Stachybotrys chartarum, Colonisation

    Mots-clés Pascal anglais : Endotoxin, Cytotoxicity test, Aspergillus, Fungi Imperfecti, Fungi, Thallophyta, Penicillium, Buildings, Construction materials, Toxin, Detection, Characterization, Pathogenic, Stachybotrys chartarum, Colonization

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 97-0165544

    Code Inist : 002A31D08. Création : 21/05/1997.