Microbial toxins and eukaryotic cell toxicity from indoor building materials heavily colonized by fungi and bacteria were analyzed.
The dominant colonizers at water-damaged sites of the building were Stachybotrys chartarum (103 to 105 visible conidia cm-2), Penicillium and Aspergillus species (104 CFU mg-1), gram-negative bacteria (104 CFU mg-1), and mycobacteria (103CFU mg-1).
The mycobacterial isolates were most similar to M. komossense, with 98% similarity of the complete 16S rDNA sequence.
Limulus assay of water extracts prepared from a water-damaged gypsum liner revealed high contents of gram-negative endotoxin (17 ng mg-1 of E. coli lipopolysaccharide equivalents) and bêta-D-glucan (210 ng mg-1of curdlan equivalents).
High-performance liquid chromatography analysis of the methanol extracts showed that the water-damaged gypsum liner also contained satratoxin (17 ng mg-1).
This methanol-extracted substance was 200 times more toxic to rabbit skin and fetus feline lung cells than extract of gypsum liner sampled from a non-water-damaged site.
The same extract contained toxin (s) that paralyzed the motility of boar spermatozoa at extremely low concentrations ; the 50% effective concentration was 0.3 mug of dry solids per ml.
This toxicity was not explainable by the amount of bacterial endotoxin, bêta-D-glucan, or satratoxin present in the same extract. (...)
Mots-clés Pascal : Endotoxine, Test cytotoxicité, Aspergillus, Fungi Imperfecti, Fungi, Thallophyta, Penicillium, Bâtiment, Matériau construction, Toxine, Détection, Caractérisation, Pathogène, Stachybotrys chartarum, Colonisation
Mots-clés Pascal anglais : Endotoxin, Cytotoxicity test, Aspergillus, Fungi Imperfecti, Fungi, Thallophyta, Penicillium, Buildings, Construction materials, Toxin, Detection, Characterization, Pathogenic, Stachybotrys chartarum, Colonization
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0165544
Code Inist : 002A31D08. Création : 21/05/1997.