Recovery of waterborne oocysts of Cryptosporidium from water samples by the membrane-filter dissolution method.
The cellulose-acetate membrane (CAM) - filter dissolution method implemented into a Millipore Glass Microanalysis system was used for recovery of Cryptosporidium parvum oocysts seeded into 25 1 of drinking water in polyethylene carboy aspirator bottles.
CAM-entrapped oocysts were detected by immunofluorescence microscopy.
From 65 to 94 oocysts/l (mean 75 oocysts/l), 34.7% overall of the inoculated oocysts, were unrecovered as determined after the water had been drained from the bottle, rinsed with 1 l of eluting fluid (EF), and CAM-filtered.
Efficiency rates of oocyst recovery ranged from 24.0% to 64.0% (mean 44.1%), without the use of EF and from 72.1% to 82.3% (mean 78.8%) when EF was used.
To ensure a high recovery efficiency of Cryptosporidium oocysts from sampled water by the CAM-filter dissolution method, it is recommended that 1 l of EF per 25 l of water be used.
Mots-clés Pascal : Cryptosporidium parvum, Sporozoa, Protozoa, Teneur, Détection, Analyse quantitative, Contamination biologique, Méthode étude, Méthodologie, Eau potable, Oocyste, Filtre membrane
Mots-clés Pascal anglais : Cryptosporidium parvum, Sporozoa, Protozoa, Content, Detection, Quantitative analysis, Biological contamination, Investigation method, Methodology, Drinking water, Oocyst, Membrane filter
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Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0164838
Code Inist : 002B11A. Création : 21/05/1997.