Primers obtained from gene sequences coding for heat shock proteins (HSP) were used to specifically detect enteric protozoans of the genus Giardia.
The HSP primers amplified Giardia DNA or the corresponding RNA sequences obtained from lysed cysts and gave a 163-bp product.
Since the presence of the product did not indicate whether the cysts were viable, these amplifications are a presence/absence test only.
In contrast, amplification of heat shock-induced mRNA utilizing the same HSP primers was indicative of viable Giardia cysts.
The limit of sensitivity of the presence/absence test was 1 cyst, whereas for the viability test it was 10 cysts.
Thus, viable Giardia cysts can be rapidly and specifically detected with great sensitivity through the use of PCR amplifications.
Mots-clés Pascal : Giardia lamblia, Diplomonadida, Protozoa, Pathogène, RNA messager, Kyste, Détection, Méthode, Réaction chaîne polymérase, Amplification, Qualité eau, Gène, Protéine choc thermique, Evaluation performance, Viabilité
Mots-clés Pascal anglais : Giardia lamblia, Diplomonadida, Protozoa, Pathogenic, Messenger RNA, Cyst, Detection, Method, Polymerase chain reaction, Amplification, Water quality, Gene, Heat shock protein, Performance evaluation, Viability
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0123271
Code Inist : 002A11A. Création : 21/05/1997.