New molecular methods for the detection of hepatitis A and Norwalk viruses in shellfish.
Outbreaks of viral enteric diseases after consumption of shellfish are a major health risk.
Methodological problems (such as toxicity for cell cultures and low viral concentrations) and the unculturability of some strains (i.e. hepatitis A virus, Norwalk virus) have made it difficult to study those viruses in the environmental samples.
Currently, the analysis of the hygienic quality of marketable shellfish is determined by the use of fecal indicator bacteria, but their reliability in determining viral pollution of shellfish is very low.
Recent biotechnology developments are providing available rapid, sensitive, and specific tools for detecting food-borne viruses in shellfish and in shellfish-growing waters.
In this paper, a review of these new molecular methods is carried out, discussing their advantages and possible applications.
Mots-clés Pascal : Virus hépatite A, Picornaviridae, Virus, Virus Norwalk, Caliciviridae, Contamination biologique, Mollusque et crustacé, Détection, Acide nucléique, Méthode, Hybridation moléculaire, Réaction chaîne polymérase, Transcription, In situ, Aliment
Mots-clés Pascal anglais : Hepatitis A virus, Picornaviridae, Virus, Norwalk virus, Caliciviridae, Biological contamination, Shellfish, Detection, Nucleic acid, Method, Molecular hybridization, Polymerase chain reaction, Transcription, In situ, Food
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0121858
Code Inist : 002A05C09. Création : 21/05/1997.