- To determine the frequency of Chlamydia trachomatis genital infection within sexual partnerships using highly sensitive polymerase chain reaction (PCR) amplification and to identify the variables that might modify transmission.
- Cross-sectional study of sexual partnerships comparing in vitro culture and PCR amplification for C trachomatis.
- Two outpatient sexually transmitted disease clinics.
- Four hundred ninety-four people in sexual partnerships attending sexually transmitted disease clinics.
- Genital infection with C trachomatis.
- DNA sequencing was performed to examine specific genotypes within and between partnerships.
Cross-sectional analysis was performed to determine characteristics associated with concordance or discordance of infection with partnerships.
- Cultures were positive for C trachomatis in 8.5% of males and 12.9% of females (P=03).
Using PCR, more infections were identified both in males (14.2%) and in females (15.8%), and the difference in infection rates analyzed by sex was no longer significant.
In 20.4% of 494 couples, at least 1 partner had PCR results positive for C trachomatis, with a concordant infection rate of 10.7%, significantly higher than the 5.5% concordant infection rate demonstrable by culture (P<. 01).
Male-female and female-male transmission frequencies were equal (68%). (...)
Mots-clés Pascal : Chlamydia trachomatis, Chlamydiaceae, Chlamydiales, Bactérie, Infection, Maladie sexuellement transmissible, Réaction chaîne polymérase, Epidémiologie, Fréquence, Diagnostic, Homme, Etats Unis, Amérique du Nord, Amérique, Appareil génital pathologie, Exploration microbiologique, Biologie moléculaire
Mots-clés Pascal anglais : Chlamydia trachomatis, Chlamydiaceae, Chlamydiales, Bacteria, Infection, Sexually transmitted disease, Polymerase chain reaction, Epidemiology, Frequency, Diagnosis, Human, United States, North America, America, Genital diseases, Microbiological investigation, Molecular biology
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0073181
Code Inist : 002B05B02H. Création : 21/05/1997.