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  1. Measurement by ICP-MS of lead in plasma and whole blood of lead workers and controls.

    Article - En anglais

    Objectives-To test a simple procedure for preparing samples for measurement of lead in blood plasma (P-Pb) and whole blood (B-Pb) by inductively coupled plasma mass spectrometry (ICP-MS), to measure P-Pb and B-Pb in lead workers and controls, and to evaluate any differences in the relation between B-Pb and P-Pb between people.

    Methods-P-Pb and B-Pb were measured by ICP-MS in 43 male lead smelter workers and seven controls without occupational exposure to lead.

    For analysis, plasma and whole blood were diluted 1 in 4 and 1 in 9, respectively, with a diluted ammonia solution containing Triton-X 100 and EDTA.

    The samples were handled under routine laboratory conditions, without clean room facilities.

    Results

    P-Pb was measured with good precision (CV=5%) even at concentrations present in the controls.

    Freeze storage of the samples had no effect on the results.

    The detection limit was 0.015 mug/l. The P-Pb was 0.15 (range 0.1-0.3) mug/l in controls and 1.2 (0.3-3.6) mug/l in lead workers, although the corresponding B-Pbs were 40 (24-59) mug/l and 281 (60-530) mug/l (1 mug Pb/l=4.8 nmol/l).

    B-Pb was closely associated with P-Pb (r=0.90).

    The association was evidently non-linear ; the ratio B-Pb/P-Pb decreased with increasing P-Pb.

    Conclusions-By means of ICP-MS and a simple dilution procedure, P-Pb may be measured accurately and with good precision down to concentrations present in controls.

    Contamination of blood at sampling and analysis is no major problem. (...)

    Mots-clés Pascal : Plomb, Métal lourd, Exposition professionnelle, Analyse quantitative, Sang, Homme, Spectrométrie ICP, Liquide biologique, Médecine travail

    Mots-clés Pascal anglais : Lead, Heavy metal, Occupational exposure, Quantitative analysis, Blood, Human, Inductive coupling plasma spectrometry, Biological fluid, Occupational medicine

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 97-0045265

    Code Inist : 002B03L05. Création : 21/05/1997.