Objectives-To test a simple procedure for preparing samples for measurement of lead in blood plasma (P-Pb) and whole blood (B-Pb) by inductively coupled plasma mass spectrometry (ICP-MS), to measure P-Pb and B-Pb in lead workers and controls, and to evaluate any differences in the relation between B-Pb and P-Pb between people.
Methods-P-Pb and B-Pb were measured by ICP-MS in 43 male lead smelter workers and seven controls without occupational exposure to lead.
For analysis, plasma and whole blood were diluted 1 in 4 and 1 in 9, respectively, with a diluted ammonia solution containing Triton-X 100 and EDTA.
The samples were handled under routine laboratory conditions, without clean room facilities.
P-Pb was measured with good precision (CV=5%) even at concentrations present in the controls.
Freeze storage of the samples had no effect on the results.
The detection limit was 0.015 mug/l. The P-Pb was 0.15 (range 0.1-0.3) mug/l in controls and 1.2 (0.3-3.6) mug/l in lead workers, although the corresponding B-Pbs were 40 (24-59) mug/l and 281 (60-530) mug/l (1 mug Pb/l=4.8 nmol/l).
B-Pb was closely associated with P-Pb (r=0.90).
The association was evidently non-linear ; the ratio B-Pb/P-Pb decreased with increasing P-Pb.
Conclusions-By means of ICP-MS and a simple dilution procedure, P-Pb may be measured accurately and with good precision down to concentrations present in controls.
Contamination of blood at sampling and analysis is no major problem. (...)
Mots-clés Pascal : Plomb, Métal lourd, Exposition professionnelle, Analyse quantitative, Sang, Homme, Spectrométrie ICP, Liquide biologique, Médecine travail
Mots-clés Pascal anglais : Lead, Heavy metal, Occupational exposure, Quantitative analysis, Blood, Human, Inductive coupling plasma spectrometry, Biological fluid, Occupational medicine
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0045265
Code Inist : 002B03L05. Création : 21/05/1997.