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  1. Analysis of methylhexahydrophthalic acid in human urine.

    Article - En anglais

    A method for analysis of urinary methylhexahydrophthalic acid (MHHP acid), a metabolite of the highly sensitising methylhexahydrophthalic anhydride, is described.

    The method is based on a double liquid-solid extraction of the MHHP acid from urine, esterification with methanol and boron trifluoride catalysis, and analysis with gas chromatography-mass spectrometry in the electron impact mode.

    The detection limit was 3 ng mL-1 and the precision between 2 and 5% in the range 34 to 340 ng mL-1.

    The method gave similar results compared to another method.

    Urine stored for five years at - 20°C lost between 38 and 86% of the MHHP acid.

    (range 16-85 ng mL-1).

    Mots-clés Pascal : Surveillance biologique, Médecine travail, Exposition professionnelle, Homme, Industrie chimique, Métabolite, Allergène, Liquide biologique, Urine, Composé monocyclique, Anhydride organique, Chromatographie phase gazeuse, Méthode couplée, Spectrométrie masse, Cyclohexane-1,2-dicarboxylique acide(méthyl), Isobenzofurane-1,3-dione(méthyl perhydro)

    Mots-clés Pascal anglais : Biological monitoring, Occupational medicine, Occupational exposure, Human, Chemical industry, Metabolite, Allergen, Biological fluid, Urine, Monocyclic compound, Organic anhydride, Gas chromatography, Coupled method, Mass spectrometry

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 97-0034268

    Code Inist : 002B03L06. Création : 21/05/1997.