The determination of urinary alkylphosphates monitors occupational exposure to organophosphorus pesticides and insecticides.
This paper describes a method for the quantitative determination of the following metabolites :
O-diethyldithiophosphate (DEDTP), and O,
The compounds are derivatized with pentafluorobenzylbromide into pentafluorobenzyl esters which are determined by gas chromatography with a capillary column of intermediate polarity and a flame photometric detector specific for phosphorus.
Before the analytical gas chromatographic stage, the urinary extracts are purified on Isolute SPE CN columns.
The proposed method has a limit of detection of 2-3 mug/L at 62.5 mug/L and a mean recovery of the purification stage of 85.8-101.0% for all six compounds.
The coefficient of variation of the analytical procedure was 7.9-11.9% for the six compounds.
The low detection limits make this method suitable also for determining the six metabolites in the general population.
Mots-clés Pascal : Analyse chimique, Analyse quantitative, Pesticide, Insecticide, Organophosphoré, Métabolite, Thiophosphate organique, Phosphate organique, Dithiophosphate organique, Polluant, Liquide biologique, Urine, Homme, Italie, Europe, Chromatographie phase gazeuse, Photomètre flamme
Mots-clés Pascal anglais : Chemical analysis, Quantitative analysis, Pesticides, Insecticide, Organophosphorus compounds, Metabolite, Organic thiophosphate, Organic phosphate, Organic dithiophosphate, Pollutant, Biological fluid, Urine, Human, Italy, Europe, Gas chromatography, Flame photometer
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0011062
Code Inist : 002B03G. Création : 21/05/1997.