Analytical method for the determination of urinary alkylphosphates in subjects occupationally exposed to organophosphorus pesticides and in the general population.
The determination of urinary alkylphosphates monitors occupational exposure to organophosphorus pesticides and insecticides.
This paper describes a method for the quantitative determination of the following metabolites :
O-diethyldithiophosphate (DEDTP), and O,
The compounds are derivatized with pentafluorobenzylbromide into pentafluorobenzyl esters which are determined by gas chromatography with a capillary column of intermediate polarity and a flame photometric detector specific for phosphorus.
Before the analytical gas chromatographic stage, the urinary extracts are purified on Isolute SPE CN columns.
The proposed method has a limit of detection of 2-3 mug/L at 62.5 mug/L and a mean recovery of the purification stage of 85.8-101.0% for all six compounds.
The coefficient of variation of the analytical procedure was 7.9-11.9% for the six compounds.
The low detection limits make this method suitable also for determining the six metabolites in the general population.
Mots-clés Pascal : Analyse chimique, Analyse quantitative, Pesticide, Insecticide, Organophosphoré, Métabolite, Thiophosphate organique, Phosphate organique, Dithiophosphate organique, Polluant, Liquide biologique, Urine, Homme, Italie, Europe, Chromatographie phase gazeuse, Photomètre flamme
Mots-clés Pascal anglais : Chemical analysis, Quantitative analysis, Pesticides, Insecticide, Organophosphorus compounds, Metabolite, Organic thiophosphate, Organic phosphate, Organic dithiophosphate, Pollutant, Biological fluid, Urine, Human, Italy, Europe, Gas chromatography, Flame photometer
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0011062
Code Inist : 002B03G. Création : 21/05/1997.