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  1. HPRT mutant frequencies and detection of large deletions by multiplex-PCR in human lymphocytes of vinyl chloride exposed and non-exposed populations.

    Article, Communication - En anglais

    European Meeting of Environmental Hygiene. Prague (CZE), 1995/06/27.

    The frequency of hypoxanthine guanine phosphoribosyltransferase (HPRT) mutations was determined in human T-lymphocytes isolated from peripheral blood of three populations : (1) 24 employees occupationally exposed to vinyl chloride monomer ; (2) 23 healthy non-exposed matched control individuals and (3) 41 regular blood donors.

    In addition, mutant clones of all studied groups were examined by multiplex-PCR for visible abnormalities of the gene (large deletions).

    Surprisingly, the mutation frequency of all three investigated populations was approximately the same (7-8 x 10-6).

    However, great differences occurred for the spectrum of mutants.

    Among the mutant clones of the non-exposed healthy individuals only 6% (blood donors) and 11% (matched control group) showed large deletions.

    The corresponding percentage of large deletions in the occupationally exposed group was, at 21%, much higher.

    Mots-clés Pascal : Toxicité, Mutagène, Carcinogène, Ethylène(chloro), Hydrocarbure chloré, Homme, Non fumeur, Fumeur, Sexe, Age, Exposition professionnelle, Médecine travail, Mutation, Analyse corrélation, Gène HPRT

    Mots-clés Pascal anglais : Toxicity, Mutagen, Carcinogen, Ethylene(chloro), Chlorocarbon, Human, Non smoker, Smoker, Sex, Age, Occupational exposure, Occupational medicine, Mutation, Correlation analysis

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 97-0002768

    Code Inist : 002B04E02. Création : 21/05/1997.