HPRT mutant frequencies and detection of large deletions by multiplex-PCR in human lymphocytes of vinyl chloride exposed and non-exposed populations.
European Meeting of Environmental Hygiene. Prague (CZE), 1995/06/27.
The frequency of hypoxanthine guanine phosphoribosyltransferase (HPRT) mutations was determined in human T-lymphocytes isolated from peripheral blood of three populations : (1) 24 employees occupationally exposed to vinyl chloride monomer ; (2) 23 healthy non-exposed matched control individuals and (3) 41 regular blood donors.
In addition, mutant clones of all studied groups were examined by multiplex-PCR for visible abnormalities of the gene (large deletions).
Surprisingly, the mutation frequency of all three investigated populations was approximately the same (7-8 x 10-6).
However, great differences occurred for the spectrum of mutants.
Among the mutant clones of the non-exposed healthy individuals only 6% (blood donors) and 11% (matched control group) showed large deletions.
The corresponding percentage of large deletions in the occupationally exposed group was, at 21%, much higher.
Mots-clés Pascal : Toxicité, Mutagène, Carcinogène, Ethylène(chloro), Hydrocarbure chloré, Homme, Non fumeur, Fumeur, Sexe, Age, Exposition professionnelle, Médecine travail, Mutation, Analyse corrélation, Gène HPRT
Mots-clés Pascal anglais : Toxicity, Mutagen, Carcinogen, Ethylene(chloro), Chlorocarbon, Human, Non smoker, Smoker, Sex, Age, Occupational exposure, Occupational medicine, Mutation, Correlation analysis
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 97-0002768
Code Inist : 002B04E02. Création : 21/05/1997.