Listeria monocytogenes strains were examined by restriction-enzyme analysis of chromosomal DNA using a total of 18 restriction enzymes.
Ten of the 6-base restriction enzymes and one 8-base restriction enzyme produced distinguishable fragments among these strains.
Six strains (serotype 1/2a) recovered from raw milk suspected of the same contaminant were compared with seven epidemiologically unrelated strains (serotype 1/2a) using 10 of the 6-base restriction enzymes.
The restriction enzyme patterns of the six raw milk isolates were identical to each other, but differed from those of the other strains.
Restriction-enzyme analysis of the chromosomal DNA of L. monocytogenes by using the 6-base restriction enzymes may be a useful method of epidemiological analysis for listeriosis outbreaks.
Mots-clés Pascal : Souche, Méthode fingerprint, Epidémiologie, Listeria monocytogenes, Bactérie, Pathogène, Isolat, Lait cru, Contrôle microbiologique, Identification
Mots-clés Pascal anglais : Strain, Fingerprint method, Epidemiology, Listeria monocytogenes, Bacteria, Pathogenic, Isolate, Raw milk, Microbiological testing, Identification
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0465154
Code Inist : 002A35B04. Création : 10/04/1997.