Einsatz der Fourier-Transformations-Infrarot-Spektroskopie zur Bestimmung von Mikroorganismen aus Reinräumen.
Traduction en anglais : Use of Fourier transformation infrared spectroscopy for the identification of microorganisms from clean rooms.
388 microorganisms isolated over a 5-month period during the environmental monitoring of two clean rooms were used to check the differentiation and identification performance of FT-IR spectroscopy.
Standard microscopic and biochemical investigations, in combination with API (Analytical Profile Index' ) differentiation systems were used for comparison purposes.
FT-IR spectroscopy was capable of differentiating 338 of the isolates, while the remaining 50 germs could only be roughly classified due to excessively light growth on the standard culture media.
However, neither FT-IR spectroscopy nor conventional differentiation techniques were able to process further these 50 germs and identify them at genus level.
The differentiation findings for the 338 isolates investigated by spectroscopy tallied with those resulting from conventional methods in 95.9% of cases.
Microbial profiles for the clean rooms were prepared on the basis of the frequency distributions observed for the identified genera.
The periodic preparation of microbial profiles is an important tool in microbiological status monitoring.
FT-IR spectroscopy has thus proved itself as a suitable tool for epidemiological investigation and the preparation of microbial profiles in clean rooms.
Mots-clés Pascal : Salle blanche, Contamination, Microorganisme, Transformation Fourier, Spectrométrie IR, Epidémiologie, Hygiène, Identification, Industrie pharmaceutique, Stérilité, Méthode étude
Mots-clés Pascal anglais : Clean room, Contamination, Microorganism, Fourier transformation, Infrared spectrometry, Epidemiology, Hygiene, Identification, Pharmaceutical industry, Sterility, Investigation method
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0446004
Code Inist : 002B30A08. Création : 10/04/1997.