A novel virus, GB virus-C (GBV-C), with a genome organization similar to those of the Flaviviridae family was identified in sera of patients diagnosed with hepatitis.
Up to now little has been known about the prevalence of GBV-C sequences in German hepatitis C virus infected patients.
We investigated two groups of patients chronically infected with hepatitis C virus : (i) Women infected in 1978/79 with HCV-contaminated anti-D immunoglobulin batches in former Eastern Germany, and (ii) I.v. drug users infected with different HCV subtypes.
Nested polymerase chain reaction products amplified with GBV-C specific primer pairs within the helicase region were sequenced directly and compared with the GBV-C sequences reported recently.
GBV-C sequences of the putative NS 3 gene region were shown to occur in two randomly selected anti-D immunoglobulin batches of 1978 (GI and GII) and two sera of HCV-infected women drawn in 1979.
In patient 3, the HCV-infected erythrocyte donor in 1978, specific GBV-C sequences were also evident in serum drawn in 1990.
In the high-risk group of i.v. drug users, 49% were GBV-C RNA positive.
Among the 21 GBV-C positive samples, 11 were coinfected with HCV subtype 3a and 10 with subtype 1 b. All isolates showed an overall homology to the GBV-C sequence reported by Simons of 75-81% at the nucleotide level and 94-100% at the amino acid level. (...)
Mots-clés Pascal : Hépatite virale C, Virose, Infection, Virus hépatite C, Flaviviridae, Virus, Association morbide, Prévalence, Contamination, Globuline anti D, Toxicomanie, Voie intraveineuse, Exploration microbiologique, Réaction chaîne polymérase, Résultat, Homme, Allemagne, Europe, Appareil digestif pathologie, Foie pathologie, Biologie moléculaire, Virus GB-C
Mots-clés Pascal anglais : Viral hepatitis C, Viral disease, Infection, Hepatitis C virus, Flaviviridae, Virus, Concomitant disease, Prevalence, Contamination, Anti D globulin, Drug addiction, Intravenous administration, Microbiological investigation, Polymerase chain reaction, Result, Human, Germany, Europe, Digestive diseases, Hepatic disease, Molecular biology
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0437946
Code Inist : 002B05C02G. Création : 10/04/1997.