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  1. Detection of enterovirus 70 by polymerase chain reaction in acute hemorrhagic conjunctivitis.

    Article - En anglais

    PURPOSE 

    We have developed and applied reverse transcription-polymerase chain reaction for the detection of enterovirus 70 from conjunctival swabs of patients among the population of an acute hemorrhagic conjunctivitis epidemic in Okinawa, Japan, in 1994.

    METHODS 

    The primers used were selected from the VP1, VP2, and VP3 regions of the enterovirus 70 nucleotide sequence.

    RESULTS 

    Twelve of 27 culture-negative specimens were positive by reverse transcription-polymerase chain reaction.

    CONCLUSIONS 

    The reverse transcription-polymerase chain reaction test provides a reliable diagnostic method that readily allows specific detection of enterovirus 70 in patients with acute hemorrhagic conjunctivitis in whom low enterovirus 70 recovery rates have been obtained recently by cell culture.

    Mots-clés Pascal : Conjonctivite hémorragique, Virose, Infection, Aigu, Enterovirus 70, Enterovirus, Picornaviridae, Virus, Diagnostic, Réaction chaîne polymérase, Japon, Asie, Homme, Oeil pathologie, Conjonctive pathologie

    Mots-clés Pascal anglais : Hemorrhagic conjunctivitis, Viral disease, Infection, Acute, Enterovirus 70, Enterovirus, Picornaviridae, Virus, Diagnosis, Polymerase chain reaction, Japan, Asia, Human, Eye disease, Conjunctiva disease

    Logo du centre Notice produite par :
    Inist-CNRS - Institut de l'Information Scientifique et Technique

    Cote : 96-0389647

    Code Inist : 002B05C02B. Création : 10/04/1997.