Detection of enterovirus 70 by polymerase chain reaction in acute hemorrhagic conjunctivitis.
We have developed and applied reverse transcription-polymerase chain reaction for the detection of enterovirus 70 from conjunctival swabs of patients among the population of an acute hemorrhagic conjunctivitis epidemic in Okinawa, Japan, in 1994.
The primers used were selected from the VP1, VP2, and VP3 regions of the enterovirus 70 nucleotide sequence.
Twelve of 27 culture-negative specimens were positive by reverse transcription-polymerase chain reaction.
The reverse transcription-polymerase chain reaction test provides a reliable diagnostic method that readily allows specific detection of enterovirus 70 in patients with acute hemorrhagic conjunctivitis in whom low enterovirus 70 recovery rates have been obtained recently by cell culture.
Mots-clés Pascal : Conjonctivite hémorragique, Virose, Infection, Aigu, Enterovirus 70, Enterovirus, Picornaviridae, Virus, Diagnostic, Réaction chaîne polymérase, Japon, Asie, Homme, Oeil pathologie, Conjonctive pathologie
Mots-clés Pascal anglais : Hemorrhagic conjunctivitis, Viral disease, Infection, Acute, Enterovirus 70, Enterovirus, Picornaviridae, Virus, Diagnosis, Polymerase chain reaction, Japan, Asia, Human, Eye disease, Conjunctiva disease
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0389647
Code Inist : 002B05C02B. Création : 10/04/1997.