Risk Assessment Issues for Sensitive Human Populations. Conference. Wright-Patterson AFB, OH USA, 1995/04/25.
Immunologic mechanisms contributing to diisocyanate-induced occupational asthma (OA) are poorly defined.
There is a relatively low incidence of diisocyanate-specific IgE antibody responses.
The frequent occurrence of delayed onset asthmatic responses in workers with diisocyanate asthma suggests a role for cellular immune mechanisms.
We have shown in vitro production of antigen-specific mononuclear cell-derived histamine releasing factors (HRF) by peripheral blood mononuclear cells (PBMCs) of workers with OA.
Monocyte chemoattractant protein-1 (MCP-1) and RANTES (acronym for « regulated on activation normal T expressed and secreted ») are chemokines found in PBMC supernatants that express HRF activity.
Diisocyanate-exposed workers were tested for diisocyanate antigen-stimulated enhancement of HRF, MCP-I, and RANTES production in supernatants of PBMCs and for serum specific IgE and IgG antibody levels to diisocyanate antigens bound to human serum albumin (HSA).
PBMCs of workers with diisocyanate OA showed significantly increased production of antigen-specific HRF activity and MCP-1 (>300 ng/ml) compared to diisocyanate-exposed asymptomatic workers (P<0.05).
Antigen-stimulated enhancement of MCP-1 mRNA was demonstrated by reverse-transcription PCR.
RANTES mRNA and chemokine secretion (<1 ng/ml) was also demonstrated in PBMCs, but did not show antigen enhancement in OA workers. (...)
Mots-clés Pascal : Diisocyanate organique, Toxicité, Composé chimique, Asthme, Exposition professionnelle, Médecine travail, Homme, Histamine, Cytokine, Facteur chimiotactique, Immunopathologie, Appareil respiratoire pathologie, Bronchopneumopathie obstructive
Mots-clés Pascal anglais : Organic diisocyanate, Toxicity, Chemical compound, Asthma, Occupational exposure, Occupational medicine, Human, Histamine, Cytokine, Chemotactic factor, Immunopathology, Respiratory disease, Obstructive pulmonary disease
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Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0381169
Code Inist : 002B06C02. Création : 10/04/1997.