Evaluation of PCR, nested PCR, and fluorescent antibodies for detection of Giardia and Cryptosporidium species in wastewater.
Giardiasis and cryptosporidiosis are diseases caused by the protozoan parasites Giardia lamblia and Cryptosporidium parvum.
Waterborne transmission of these organisms has become more prevalent in recent years, and regulatory agencies are urging that source and finished water be screened for these organisms.
A major problem associated with testing for these organisms is the lack of reliable methodologies and baseline information on the prevalence of these parasites in various water sources.
Our study addressed both of these issues.
We evaluated the presence and reduction of Giardia cysts and Cryptosporidium oocysts in sewage effluent by a combination of indirect fluorescent antibody (IFA) staining and PCR.
Our results indicated a 3-log reduction of Giardia cysts and a 2-log reduction of Cryptosporidium oocysts through the sewage treatment process as determined by IFA.
We developed a nested PCR to detect Cryptosporidium oocysts and used a double PCR to detect Giardia cysts.
A 100% correlation was noted between IFA and PCR detection of Giardia cysts while correlation for Cryptosporidium oocysts was slightly less.
On the basis of these results, PCR may be a useful tool in the environmental analysis of water samples for Giardia and Cryptosporidium organisms.
Mots-clés Pascal : Cryptosporidium parvum, Sporozoa, Protozoa, Giardia lamblia, Diplomonadida, Eau usée, Contamination biologique, Détection, Réaction chaîne polymérase, Anticorps, Marquage fluorescent, Méthode, Evaluation performance
Mots-clés Pascal anglais : Cryptosporidium parvum, Sporozoa, Protozoa, Giardia lamblia, Diplomonadida, Waste water, Biological contamination, Detection, Polymerase chain reaction, Antibody, Fluorescent labelling, Method, Performance evaluation
Notice produite par :
Inist-CNRS - Institut de l'Information Scientifique et Technique
Cote : 96-0344697
Code Inist : 002A11A. Création : 10/04/1997.